Poliovirus: generation and characterization of mutants.
Poliovirus: generation and characterization of mutants.
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DOI：
10.1002/9780471729259.mc15h02s29
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发表时间：
2013
影响因子：
--
通讯作者：
Andino R
中科院分区：
文献类型：
--
作者：
Burrill CP;Strings VR;Schulte MB;Andino R
脊髓灰质炎病毒（PV）是典型的小核糖核酸病毒。它是一种非包膜RNA病毒，具有小（约7.5 kb）的正极性基因组。几种菌株的cDNA克隆是可用的，并且传染性病毒可以通过在体外转录的病毒基因组转染到适当的宿主细胞中产生。脊髓灰质炎病毒基因研究的便利性是其长期作为研究RNA病毒生物学、发病机制和进化模型的主要原因。提出了PV突变体产生和表征的方案。关于PV的生产，繁殖，定量和纯化的单独单元也将被提出。
Poliovirus (PV) is the prototypical picornavirus. It is a non-enveloped RNA virus with a small (~7.5 kb) genome of positive polarity. cDNA clones of several strains are available, and infectious virus can be produced by the transfection of in vitro transcribed viral genomes into an appropriate host cell. The ease of genetic studies in poliovirus is a primary reason that it has long served as a model to study RNA virus biology, pathogenesis, and evolution. Protocols for the generation and characterization of PV mutants are presented. A separate unit concerning the production, propagation, quantification, and purification of PV will also be presented.