Biodelignification of rice straw by Phanerochaete chrysosporium in the presence of dirhamnolipid
Biodelignification of rice straw by Phanerochaete chrysosporium in the presence of dirhamnolipid
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地鼠脂存在下黄孢原毛平革菌对稻秆的生物脱木质素
DOI：
10.1007/s10532-010-9329-0
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发表时间：
2010-07-01
期刊：
影响因子：
3.6
通讯作者：
Zhao, Jia-Jia
中科院分区：
文献类型：
--
作者：
Liang, Yun-Shan;Yuan, Xing-Zhong;Zhao, Jia-Jia
白腐真菌降解木质素作为减少木质纤维素废物在环境中积累的一种手段已经受到相当大的关注。表面活性剂对真菌木质纤维素生物转化的促进作用也引起了广泛的关注。研究了二鼠李糖脂生物表面活性剂对黄孢原毛平革菌降解稻草的影响。结果表明，二鼠李糖脂生物表面活性剂的存在可显著促进稻草的生物脱木素过程。特别地，浓度为0.007%的二鼠李糖脂使木质素过氧化物酶（LiP）的峰值活性增加了86%，而不影响锰过氧化物酶（MnP）的活性。二鼠李糖脂能有效提高秸秆基质中水溶性有机碳（WSOC）的含量，促进微生物的生长和活性，当二鼠李糖脂添加量为0.007%时，木质素的降解率提高了54%。观察到的化学结构和形态的变化表明，秸秆基质脱木质素在二鼠李糖脂的存在下，与内部细胞纤维和简单的化合物的释放分离的梯田状片段的形成。变异分配分析表明，添加二鼠李糖脂诱导了显著的秸秆生物脱木质化，解释了22.1%（P = 0.013）的方差。
Lignin degradation by white-rot fungi has received considerable attention as a means for reducing accumulation of lignocellulosic wastes in the environment. The stimulatory effect of surfactants on fungal lignocellulose bioconversion also has attracted wide interest. In this study the influence of dirhamnolipid biosurfactant on biodegradation of rice straw by Phanerochaete chrysosporium was investigated. It was shown that the biodelignification process of rice straw can be significantly enhanced by the presence of dirhamnolipid biosurfactant. In particular, the dirhamnolipid at the concentration of 0.007% increased the peak activity of lignin peroxidase (LiP) by 86% without affecting the manganese peroxidase (MnP) activity. The water-soluble organic carbon (WSOC) contents in the straw substrates as well as the microbial growth and activity were effectively improved by dirhamnolipid, while the degradation rate of lignin increased by 54% with dirhamnolipid of 0.007%. Observed chemical structural and morphological changes showed that the straw substrates were delignified in the presence of dirhamnolipid with the formation of terrace-like fragments separated from the inner cellular fibers and the release of simple compounds. Variation partitioning analysis revealed that the dirhamnolipid addition induced a significant straw biodelignification which explained 22.1% (P = 0.013) of the variance.