Assessment of the predictive value of follicular fluid insulin, leptin and adiponectin in assisted reproductive cycles
Assessment of the predictive value of follicular fluid insulin, leptin and adiponectin in assisted reproductive cycles
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DOI：
10.3109/09513591003632050
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发表时间：
2010-07-01
影响因子：
2
通讯作者：
Kikkawa, Fumitaka
中科院分区：
文献类型：
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作者：
Takikawa, Sachiko;Iwase, Akira;Kikkawa, Fumitaka
目的。目的探讨卵泡内胰岛素、瘦素和脂联素水平与辅助生殖技术（ART）结果的相关性。这是一项对46例接受体外受精/胞浆内单精子注射的患者的回顾性研究。采用酶联免疫吸附法检测卵母细胞回收时收集的卵泡液（FF）中胰岛素、瘦素和脂联素的水平，并分析其与ART疗效的相关性。滤泡内胰岛素、瘦素和脂联素水平无显著相关性。在妊娠（n = 20）和非妊娠（n = 26）周期之间，胰岛素浓度有显著差异（P = 0.007），但瘦素和脂联素浓度无显著差异（P = 0.007）。胰岛素浓度大于7 mU/l的12个周期中仅有2例妊娠，而胰岛素浓度小于7 mU/l的34个周期中有18例妊娠（P = 0.043）。在多囊卵巢综合征（PCOS）患者的非妊娠周期中，FF中胰岛素浓度明显升高。我们的结果提示卵泡内胰岛素可能参与卵泡形成。胰岛素抵抗相关物质可能影响多囊卵巢综合征患者的生殖过程。
Purpose. To assess the correlation of intrafollicular insulin, leptin and adiponectin levels with assisted reproductive technologies (ART) outcome.Methods. This was a retrospective study of 46 patients undergoing in vitro fertilisation/intracytoplasmic sperm injection. Follicular fluid (FF) samples collected at oocyte retrieval were assayed for insulin, leptin and adiponectin levels using enzyme-linked immunosorbent assay, and correlations with ART outcome were analysed.Results. There was no significant correlation between intrafollicular insulin, leptin and adiponectin levels. There was a significant difference in the concentration of insulin (P = 0.007), but not leptin or adiponectin, between pregnant (n = 20) and non-pregnant (n = 26) cycles. Only two pregnancies was observed in the 12 cycles in which the concentration of insulin was greater than 7 mU/l in FF, while 18 pregnancies was observed in the 34 cycles in which the concentration of insulin was less than 7 mU/l (P = 0.043). The significantly high concentration of insulin in FF was observed in non-pregnant cycles of patients with polycystic ovary syndrome (PCOS).Conclusions. Our results suggest the possible involvement of intrafollicular insulin in folliculogenesis. Insulin resistance-related substances may affect the reproductive process in patients with PCOS.