Multielectrode arrays with elastomeric microstructured overlays for extracellular recordings from patterned neurons
Multielectrode arrays with elastomeric microstructured overlays for extracellular recordings from patterned neurons
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DOI：
10.1088/1741-2560/2/2/l01
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发表时间：
2005-06-01
影响因子：
4
通讯作者：
Cabestany, J.
中科院分区：
文献类型：
--
作者：
Claverol-Tinture, E.;Ghirardi, M.;Cabestany, J.
多电极阵列技术构成了一个很有前途的方法，在神经系统中的信息处理的活动依赖性神经元可塑性的表征。为此，长期监测和刺激培养的神经元网络与一对一的神经元传感器接口是有利的。满足这些规格的现有神经芯片已经使用了需要洁净室密集型微制造技术的定制3D结构。具有大规模生产潜力的低成本制造程序将促进这一领域的进展。为此，我们开发了一种三明治结构，包括通过复制模制和微孔冲压进行微结构化的弹性体薄膜，用于神经元图案化，以及用于刺激和记录的标准平面微电极阵列（MEA）。弹性体膜包括用于细胞体限制的微孔和能够引导神经突用于网络拓扑规范的微通道。该装置通过将弹性体结构覆盖在平面阵列上而形成。复制品成型，快速原型和平面MEA的组合导致大多数神经生理学实验室可以获得低成本的神经芯片。单个神经元的图案和记录的细胞外电位的证明。
Multielectrode array technology constitutes a promising approach for the characterization of the activity-dependent neuronal plasticity underlying information processing in the nervous system. For this purpose, long-term monitoring and stimulation of cultured neuronal networks with one-to-one neuron-sensor interfacing is advantageous. Existing neurochips that meet these specifications have made use of custom 3D structures requiring clean-room intensive microfabrication techniques. Low-cost fabrication procedures with potential for mass production would facilitate progress in the area. To this end, we have developed a sandwich structure comprising an elastomeric film, microstructured by replica moulding and microhole punching, for neuronal patterning, and a standard planar microelectrode array (MEA), for stimulation and recording. The elastomeric film includes microwells for cell body confinement, and microchannels capable of guiding neurites for network topology specification. The device is formed by overlaying the elastomeric structures on planar arrays. The combination of replica moulding, rapid prototyping and planar MEAs results in low-cost neurochips accessible to most neurophysiology labs. Single neuron patterning and recordings of extracellular potentials are demonstrated.