Morphometrical evaluation of the choriocapillaris imaged by swept-source optical coherence tomography angiography.
Morphometrical evaluation of the choriocapillaris imaged by swept-source optical coherence tomography angiography.
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DOI：
10.2147/opth.s179634
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Ojima A
中科院分区：
文献类型：
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作者：
Sugano Y;Sekiryu T;Furuta M;Tomita R;Shintake H;Maehara H;Ojima A
目的评估扫描源光学相干断层扫描血管成像（SS-OCTA）绒毛膜定量形态学评价的可重复性。这个观察性的横断面病例系列包括健康个体的35只眼睛和32名患者的32只眼睛。使用SS-OCTA以3×3 mm平方拍摄两张中央凹图像。使用开源软件“AngioTool”对以中央凹为中心的800×800像素正方形内的绒毛膜进行形态计量学分析，该软件采用高斯递归滤波器和多尺度Hessian增强。该程序的血管厚度和强度参数可以改变，以帮助血管检测。我们测量了不同参数集的每只眼睛的图像对，并计算了形态计量结果的类内相关性（ICC）。在确定产生高再现性的参数后，我们评估了中心凹内800×800像素毫米平方的区域变化。血管面积、血管总长度、血管直径指数、平均腔隙度的ICCs均大于0.9，以“血管厚度”3-4和强度15为参数。使用相同的参数值进行测量时，血管密度和平均血管直径指数分别为60.5%和19.1±0.389。血管密度、血管长度、血管直径指数和平均腔隙度在以中心凹为中心的800×800像素方内没有显著变化，但在中心凹中心的200×200像素方除外。利用多尺度Hessian增强的开源软件进行形态计量学评价，SS-OCTA绒毛膜图像具有较高的再现性。这种自动化的形态计量分析可以对绒毛膜毛细血管提供客观的评价。
To assess the reproducibility of quantitative morphometrical evaluation of the choriocapillaris imaged with swept-source optical coherence tomography angiography (SS-OCTA). This observational, cross-sectional case series included 35 eyes of healthy individuals and 32 eyes of 32 patients. Two images of the fovea were taken using SS-OCTA with 3×3 mm squares. Images of the choriocapillaris within 800×800 pixel squares centered at the fovea were analyzed morphometrically using open-source software “AngioTool” that applies a Gaussian recursive filter and multiscale Hessian enhancement. This program’s vessel thickness and intensity parameters can be changed to aid vessel detection. We measured the pairs of images per eye with different parameter sets and calculated the intraclass correlation (ICC) for the morphometrical results. After determining the parameters that produced high reproducibility, we evaluated regional variations in 800×800 pixel mm squares within the fovea. The ICCs for vessel area, total vessel length, vessel diameter index, and mean lacunarity were over 0.9 using the parameters of “vessel thickness” 3–4 and intensity 15 in the group including all subjects. When measurements were performed using these same parameter values, the vessel density and mean vessel diameter index were 60.5% and 19.1±0.389, respectively. Vessel density, vessel length, vessel diameter index, and mean lacunarity did not change significantly within an 800×800 pixel square centered at the fovea except for the 200×200 pixel square at the foveal center. SS-OCTA images of the choriocapillaris can be measured with high reproducibility by morphometrical evaluation using open-source software with multiscale Hessian enhancement. Such automated morphometric analysis can provide an objective evaluation of the choriocapillaris.