Changes in the diversity and composition of gut microbiota of weaned piglets after oral administration of Lactobacillus or an antibiotic
Changes in the diversity and composition of gut microbiota of weaned piglets after oral administration of Lactobacillus or an antibiotic
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DOI：
10.1007/s00253-016-7845-5
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发表时间：
2016-10
影响因子：
5
通讯作者：
Dongyan Zhang;H. Ji;Hui Liu;Sixin Wang;Jing Wang;Yamin Wang
中科院分区：
文献类型：
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作者：
Dongyan Zhang;H. Ji;Hui Liu;Sixin Wang;Jing Wang;Yamin Wang
肠道微生物群在动物的健康和福祉中发挥着重要作用，高通量测序有助于探索动物肠道中的微生物种群。然而，之前的研究主要集中在粪便样本而不是胃肠道样本上。在本研究中，我们使用高通量测序比较了用罗伊氏乳杆菌或金霉素（金霉素）处理的断奶仔猪肠道内容物的微生物群多样性和组成。将9头断奶仔猪随机分为3组，分别补充罗伊氏乳杆菌、金霉素或生理盐水10天，然后获取3个肠段（空肠、结肠和盲肠）的内容物，用于16S rRNA基因V3-V4高变区的测序。在三种处理中，空肠中的微生物群多样性和组成与结肠和盲肠中的微生物群不同。在空肠中，与金霉素组相比，罗伊氏乳杆菌处理增加了微生物群的物种丰富度，如 ACE 和 Chao1 指数所示，金霉素组消除了几个分类单元。在结肠和盲肠中，金霉素组中厚壁菌门和普氏菌属的相对丰度高于其他组。聚类样本之间的距离显示，对于空肠样本，罗伊氏乳杆菌组比对照组更接近金霉素组，而罗伊氏乳杆菌组的结肠和盲肠样本与对照组的样本聚类。这项研究为未来的研究提供了基础知识，例如抗生素替代品的开发。
The gut microbiota plays important roles in the health and well-being of animals, and high-throughput sequencing facilitates exploration of microbial populations in the animal gut. However, previous studies have focused on fecal samples instead of the gastrointestinal tract. In this study, we compared the microbiota diversity and composition of intestinal contents of weaned piglets treated with Lactobacillus reuteri or chlortetracycline (aureomycin) using high-throughput sequencing. Nine weaned piglets were randomly divided into three groups and supplemented with L. reuteri, chlortetracycline, or saline for 10 days, and then the contents of three intestinal segments (jejunum, colon, and cecum) were obtained and used for sequencing of the V3–V4 hypervariable region of the 16S rRNA gene. The microbiota diversity and composition in the jejunum were different from those in the colon and cecum among the three treatments. In the jejunum, treatment with L. reuteri increased the species richness of the microbiota, as indicated by the ACE and Chao1 indexes, compared with the chlortetracycline group, in which several taxa were eliminated. In the colon and cecum, relative abundances of the phylum Firmicutes and the genus Prevotella were higher in the chlortetracycline group than in the other groups. Distances between clustered samples revealed that the L. reuteri group was closer to the chlortetracycline group than the control group for jejunum samples, while colon and cecum samples of the L. reuteri group were clustered with those of the control group. This study provides fundamental knowledge for future studies such as the development of alternatives to antibiotics.