THE INFLUENCE OF EPITHELIUM ON THE RESPONSIVENESS OF GUINEA-PIG ISOLATED TRACHEA
THE INFLUENCE OF EPITHELIUM ON THE RESPONSIVENESS OF GUINEA-PIG ISOLATED TRACHEA
复制标题
DOI：
10.1111/j.1476-5381.1986.tb10192.x
复制
发表时间：
1986-03-01
影响因子：
7.3
通讯作者：
HOLROYDE, MC
中科院分区：
文献类型：
--
作者：
HOLROYDE, MC
1本研究旨在探讨气管上皮产生类似于血管组织内皮源性舒张因子（EDRF）的舒张因子的可能性。在哮喘等疾病中，上皮损伤后缺乏这种因子可能有助于解释这类疾病的气道高反应性特征。2摩擦去除上皮可增强豚鼠离体气管链对5-羟色胺、组胺、乙酰胆碱、腺苷、异丙肾上腺素的敏感性，对KCl的敏感性也有轻微的增强。对LaCl 3和电场刺激的反应不受影响。低浓度的腺苷只在剥脱上皮的组织中产生收缩。3在吲哚美辛1.4 μ M或二硫苏糖醇（DTT）1 μ M的存在下，在对照和摩擦组织中，对组胺的剂量-反应曲线移向左侧，并且最大反应增加。有上皮和无上皮组织之间的敏感性差异不受吲哚美辛的影响，但DTT略有减少。Phenidone（0.1 mM）也增加了最大响应，但仅增加了具有完整上皮的组织的敏感性，达到与剥脱上皮的组织中所见的相同水平。4灌流级联研究未提供气管上皮产生松弛因子的证据。[5]这表明，去除上皮细胞产生的超敏反应不是由于去除了松弛因子，而是由于去除了渗透屏障，从而使底层平滑肌水平的激动剂浓度更高。
1 This study was designed to investigate the possibility that tracheal epithelium generates a relaxant factor analogous to the endothelial-derived relaxant factor (EDRF) of vascular tissue. The absence of such a factor following epithelial damage in diseases such as asthma might help to explain the airway hyperreactivity characteristic of such diseases. 2 Removal of epithelium by rubbing enhanced the sensitivity of guinea-pig isolated tracheal chains to 5-hydroxytryptamine histamine, acetylcholine, adenosine, isoprenaline and also minimally to KCl. Responses to LaCl3 and electrical field stimulation were not affected. Low concentrations of adenosine produced contractions only in tissues denuded of epithelium. 3 In the presence of indomethacin 1.4 .mu.M or dithiothreitol (DTT) 1 .mu.M, dose-response curves to histamine were removed to the left in both control and rubbed tissues, and the maximum response was increased. The difference in sensitivity between tissues with and without epithelium was not affected by indomethacin, but was slightly reduced by DTT. Phenidone (0.1 mM) also increased the maximum responses, but increased the sensitivity only of the tissues with intact epithelium, to the same level as that seen in the tissues denuded of epithelium. 4 Superfusion cascade studies provided no evidence for the generation of a relaxant factor from tracheal epithelium. 5 It is suggested that the supersensitivity produced by removal of the epithelium is not due to the removal of the relaxant factor, but rather to the removal of a permeability barrier, allowing a greater concentration of agonist at the level of the underlying smooth muscle.