Direct Regulation of Extracellular Chitinase Production by the Transcription Factor LeClp in Lysobacter enzymogenes OH11
Direct Regulation of Extracellular Chitinase Production by the Transcription Factor LeClp in Lysobacter enzymogenes OH11
复制标题
溶杆菌 OH11 中转录因子 LeClp 直接调节细胞外几丁质酶的产生
DOI：
10.1094/phyto-01-16-0001-r
复制
发表时间：
2016-09-01
期刊：
影响因子：
3.2
通讯作者：
Liu, Fengquan
中科院分区：
文献类型：
--
作者：
Xu, Huiyong;Chen, Hongfu;Liu, Fengquan
产酶溶杆菌是一种革兰氏阴性细菌生物防治剂，其产生的大量胞外酶能够降解真菌病原体的细胞壁。在OH11菌株中，LeClp通过调控几丁质酶编码基因chiA的转录来控制胞外几丁质酶的产生。使用生物信息学，遗传学和生物化学方法的组合，我们表明，LeClp调节chiA转录直接结合到chiA启动子区。虽然LeClp似乎在这一作用中很重要，但它不是chiA转录的唯一调节因子。此外，推定的LeClp结合位点的序列分析表明，LeClp同源物可以参与调节胞外几丁质酶的生产在不同的溶杆菌属。通过类似于L.酶基因我们的研究结果提出了新的见解LeClp在控制胞外几丁质酶活性的分子机制，提供了一个基本的道路，以阐明LeClp如何调节L enzymogenes的其他胞外裂解酶的生产。
Lysobacter enzymogenes is a gram-negative bacterial biological control agent that produces abundant extracellular enzymes capable of degrading the cell walls of fungal pathogens. In strain OH11, an isolate from China, the global regulator LeClp controls the production of extracellular chitinase by regulating the transcription of the chitinase-encoding gene chiA. Using a combination of bioinformatic, genetic, and biochemical methods, we show that LeClp regulates chiA transcription by directly binding to the chiA promoter region. Although LeClp appears to be important in this role, it is not the sole regulator of chiA transcription. Furthermore, the sequence analysis of putative LeClp binding sites indicated that the LeClp homolog could be involved in the regulation of extracellular chitinase production in diverse Lysobacter spp. by a mechanism similar to that in L. enzymogenes. Our findings present new insights into the molecular mechanism of LeClp in controlling extracellular chitinase activity, providing a fundamental road to elucidate how LeClp regulates the production of other extracellular lytic enzymes in L enzymogenes.