Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization
Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization
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DOI：
10.1016/s0092-8674(04)00448-9
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发表时间：
2004-05-14
期刊：
影响因子：
64.5
通讯作者：
Silver, PA
中科院分区：
文献类型：
--
作者：
Casolari, JM;Brown, CR;Silver, PA
与核孔复合体(NPC)和核运输因子相关的基因被认为与转录调控有关。因此，我们研究了核转运机制的组件，包括核粘附素、核孔蛋白和Ran鸟嘌呤核苷酸交换因子(Rangef)与酿酒酵母基因组的关联。我们发现，大多数核孔蛋白和核粘附素优先与高转录基因的子集和具有Rap1结合位点的基因相关联，而Rangef优先与转录不活跃的基因相关联。与转录与核孔的耦合一致，我们发现Gal基因的转录激活导致它们与核孔蛋白结合，重新定位到核周，并失去Rangef结合。综上所述，这些结果表明基因组的组织通过转录状态与核运输机制耦合。
The association of genes with the nuclear pore complex (NPC) and nuclear transport factors has been implicated in transcriptional regulation. We therefore examined the association of components of the nuclear transport machinery including karyopherins, nucleoporins, and the Ran guanine-nucleotide exchange factor (RanGEF) with the Saccharomyces cerevisiae genome. We find that most nucleoporins and karyopherins preferentially associate with a subset of highly transcribed genes and with genes that possess Rap1 binding sites whereas the RanGEF preferentially associates with transcriptionally inactive genes. Consistent with coupling of transcription to the nuclear pore, we show that transcriptional activation of the GAL genes results in their association with nuclear pore proteins, relocation to the nuclear periphery, and loss of RanGEF association. Taken together, these results indicate that the organization of the genome is coupled via transcriptional state to the nuclear transport machinery.