Three-dimensional motion tracking for high-resolution optical microscopy, in vivo.
Three-dimensional motion tracking for high-resolution optical microscopy, in vivo.
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DOI：
10.1111/j.1365-2818.2012.03613.x
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发表时间：
2012-06
影响因子：
2
通讯作者：
Balaban RS
中科院分区：
文献类型：
--
作者：
Bakalar M;Schroeder JL;Pursley R;Pohida TJ;Glancy B;Taylor J;Chess D;Kellman P;Xue H;Balaban RS
在进行光学成像实验时，在体内，组织的生理运动从根本上限制了信噪比和有效的时空分辨率。描述了一种三维运动跟踪方案，该方案使用带有谐振振镜的多光子激发显微镜(512×512像素，33帧/秒)来克服活体中的生理运动。商业上可获得的图形处理单元的使用允许连续体积的快速三维互相关，以检测位移并调整组织位置以接近实时地跟踪运动。运动体模测试保持了微米分辨率，位移速度高达200Mm/μ，完全在许多生物组织在生理相关条件下观察到的漂移范围内。在小鼠骨骼肌的活体实验中，使用以腔染料为位移参照物的毛细血管系统揭示了一种有效而稳健的跟踪组织运动的方法，以实现(1)信号在不影响分辨率的情况下随时间平均，以及(2)在生理扰动期间跟踪细胞区域。
When conducting optical imaging experiments, in vivo, the signal to noise ratio and effective spatial and temporal resolution is fundamentally limited by physiological motion of the tissue. A 3-dimensional motion tracking scheme, using a multi-photon excitation microscope with a resonant galvanometer, (512×512 pixels at 33 frames/sec) is described to overcome physiological motion, in vivo. The use of commercially available graphical processing units permitted the rapid 3-dimensional cross-correlation of sequential volumes to detect displacements and adjust tissue position to track motions in near real-time. Motion phantom tests maintained micron resolution with displacement velocities of up to 200 μm/min, well within the drift observed in many biological tissues under physiologically relevant conditions. In vivo experiments on mouse skeletal muscle using the capillary vasculature with luminal dye as a displacement reference revealed an effective and robust method of tracking tissue motion to enable (1) signal averaging over time without compromising resolution, and (2) tracking of cellular regions during a physiological perturbation.
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影响因子：
20.1
作者：
Schroeder, James L.;Luger-Hamer, Merav;Balaban, Robert S.
通讯作者：
Balaban, Robert S.
影响因子：
3.4
作者：
Rothstein, EC;Carroll, S;Balaban, RS
通讯作者：
Balaban, RS
影响因子：
19.5
作者：
Hermosillo, G;Chefd'Hotel, C;Faugeras, O
通讯作者：
Faugeras, O
影响因子：
5.5
作者：
Dunn, KW;Sandoval, RM;Molitoris, BA
通讯作者：
Molitoris, BA
影响因子：
2
作者：
Rothstein, Emily C.;Nauman, Michael;Balaban, Robert S.
通讯作者：
Balaban, Robert S.