Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance
Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance
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DOI：
10.1016/j.nutres.2010.10.009
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发表时间：
2010-12-01
影响因子：
4.5
通讯作者：
Fukuda, Shigeharu
中科院分区：
文献类型：
--
作者：
Arai, Chikako;Arai, Norie;Fukuda, Shigeharu
在人体口服糖耐量试验中，海藻糖引起的胰岛素分泌低于葡萄糖。鉴于海藻糖的这种低胰岛素血症效应，我们假设海藻糖通过减少甘油三酯的储存来抑制脂肪细胞肥大，并减轻高脂饮食（HFD）小鼠的胰岛素抵抗。给小鼠喂食HFD，并随意给予含有2.5%糖（葡萄糖[Glc]、海藻糖[Tre]、麦芽糖[Mal]、高果糖玉米糖浆或果糖[Fru]）的饮用水。在HFD和糖摄入7周后，Tre/HFD组的空腹血清胰岛素水平显著低于Mal/HFD和Glc/HFD组（P <0.05）。此外，与Mal/HFD组相比，Tre/HFD组显示出显著抑制的稳态模型评估胰岛素抵抗的升高（P <0.05），并且显示出比Glc/HFD组更低的稳态模型评估胰岛素抵抗的趋势。喂养8周后，Tre/HFD组的肠系膜脂肪细胞大小显示出比Glc/HFD、Mal/HFD、高果糖玉米糖浆/HFD或Fru/HFD组显著更小的肥大。肠系膜脂肪细胞基因表达分析显示，在Tre/HFD组和蒸馏水/标准饮食组之间观察到单核细胞趋化蛋白-1（MCP-1）信使RNA（mRNA）表达无统计学显著差异，而在Glc/HFD、Mal/HFD、Fru/HFD和蒸馏水/HFD组中观察到MCP-1 mRNA表达显著增加。因此，我们的数据表明，海藻糖通过减少胰岛素分泌和下调MCP-1的mRNA表达来防止脂肪细胞肥大并减轻HFD喂养小鼠的胰岛素抵抗。这些发现进一步表明海藻糖是一种功能性糖，可减轻胰岛素抵抗。(C)2010年爱思唯尔公司All rights reserved.
Trehalose has been shown to evoke lower insulin secretion than glucose in oral saccharide tolerance tests in humans. Given this hypoinsulinemic effect of trehalose, we hypothesized that trehalose suppresses adipocyte hypertrophy by reducing storage of triglyceride and mitigates insulin resistance in mice fed a high-fat diet (HFD). Mice were fed an HFD and given drinking water containing 2.5% saccharide (glucose [Glc], trehalose [Tre], maltose [Mal], high-fructose corn syrup, or fructose [Fru]) ad libitum. After 7 weeks of HFD and saccharide intake, fasting serum insulin levels in the Tre/HFD group were significantly lower than in the Mal/HFD and Glc/HFD groups (P < .05). Furthermore, the Tre/HFD group showed a significantly suppressed elevation of homeostasis model assessment insulin resistance compared with the Mal/HFD group (P < .05) and showed a trend toward lower homeostasis model assessment insulin resistance than the Glc/HFD group. After 8 weeks of feeding, mesenteric adipocyte size in the Tre/HFD group showed significantly less hypertrophy than the Glc/HFD, Mal/HFD, high-fructose corn syrup/HFD, or Fru/HFD group. Analysis of gene expression in mesenteric adipocytes showed that no statistically significant difference in the expression of monocyte chemoattractant protein-1 (MCP-1) messenger RNA (mRNA) was observed between the Tre/HFD group and the distilled water/standard diet group, whereas a significant increase in the MCP-1 mRNA expression was observed in the Glc/HFD, Mal/HFD, Fru/HFD, and distilled water/HFD groups. Thus, our data indicate that trehalose prevents adipocyte hypertrophy and mitigates insulin resistance in HFD-fed mice by reducing insulin secretion and down-regulating mRNA expression of MCP-1. These findings further suggest that trehalose is a functional saccharide that mitigates insulin resistance. (C) 2010 Elsevier Inc. All rights reserved.