Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain
Whole-Genome Sequencing of Staphylococcus aureus Strain RN4220, a Key Laboratory Strain Used in Virulence Research, Identifies Mutations That Affect Not Only Virulence Factors but Also the Fitness of the Strain
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DOI：
10.1128/jb.00027-11
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发表时间：
2011-05-01
影响因子：
3.2
通讯作者：
Cheung, Ambrose L.
中科院分区：
文献类型：
--
作者：
Nair, Dhanalakshmi;Memmi, Guido;Cheung, Ambrose L.
金黄色葡萄球菌RN4220是一种克隆中间体，有时用于毒力、耐药性和代谢研究。使用全基因组测序，我们发现RN4220不同于NCTC8325，并且包含许多影响毒力和一般适合性的基因多态，这意味着在使用该毒株进行此类研究时需要谨慎。
Staphylococcus aureus RN4220, a cloning intermediate, is sometimes used in virulence, resistance, and metabolic studies. Using whole-genome sequencing, we showed that RN4220 differs from NCTC8325 and contains a number of genetic polymorphisms that affect both virulence and general fitness, implying a need for caution in using this strain for such studies.