The phylogeny of Sodalis-like symbionts as reconstructed using surface-encoding loci.
The phylogeny of Sodalis-like symbionts as reconstructed using surface-encoding loci.
复制标题
使用表面编码基因座重建的类 Sodalis 共生体的系统发育。
DOI：
10.1111/j.1574-6968.2011.02221.x
复制
发表时间：
2011
影响因子：
2.1
通讯作者：
Rio,RitaVM
中科院分区：
文献类型：
--
作者：
Snyder,AnnaK;McMillen,CynthiaM;Wallenhorst,Peter;Rio,RitaVM
16S rRNA 的系统发育分析支持 Gammaproteobacteria Sodalis gllossinidius（一种采采蝇（双翅目：Glossinidae）共生体）与感染不同昆虫目的细菌之间的密切关系。为了进一步研究这些类 Sodalis 共生体的进化关系，为假定的表面编码基因的子集（即 ompA、spr、slyB、rcsF、ycfM 和 ompC）构建了系统发育树。 TheompA 和ompC 位点分别用于检查采采蝇中Sodalis 的种内和种间多样性。 ompA 的种内分析支持非同义 (dN) 多态性升高，且单态性过多，表明选择多样化，特别是在采采舌藻 (tsetseGlossina morsitans) 内。此外，Sodalis和大肠杆菌之间的种间CompC比较表明偏离中性，在与细胞外环相关的位点观察到较高的固定值。表面编码基因在 Sodalis 和相关细菌的系统发育分辨率上有所不同，表明保守作用与宿主特异性作用。此外，Sodalis 及其近亲在 thercsF、ompA 和 ompC 位点表现出遗传分歧，表明初始分子分歧。讨论了外膜基因作为标记的应用，以进一步描述最近分化的细菌的系统学。这些结果增加了我们对昆虫共生体进化的理解，同时也确定了微生物与真核宿主整合时发生的早期基因组改变。
Phylogenetic analyses of 16S rRNA support close relationships between theGammaproteobacteria Sodalis glossinidius, a tsetse (Diptera: Glossinidae) symbiont, and bacteria infecting diverse insect orders. To further examine the evolutionary relationships of theseSodalis-like symbionts, phylogenetic trees were constructed for a subset of putative surface-encoding genes (i.e.ompA,spr, slyB,rcsF,ycfM, andompC). TheompA andompC loci were used toward examining the intra- and interspecific diversity ofSodaliswithin tsetse, respectively. Intraspecific analyses ofompA support elevated nonsynonymous (dN) polymorphism with an excess of singletons, indicating diversifying selection, specifically within the tsetseGlossina morsitans. Additionally, interspecificompC comparisons betweenSodalisandEscherichia colidemonstrate deviation from neutrality, with higher fixeddNobserved at sites associated with extracellular loops. Surface-encoding genes varied in their phylogenetic resolution ofSodalisand related bacteria, suggesting conserved vs. host-specific roles. Moreover,Sodalisand its close relatives exhibit genetic divergence at thercsF,ompA, andompC loci, indicative of initial molecular divergence. The application of outer membrane genes as markers for further delineating the systematics of recently diverged bacteria is discussed. These results increase our understanding of insect symbiont evolution, while also identifying early genome alterations occurring upon integration of microorganisms with eukaryotic hosts.