Characterization and expression profile of CaNAC2 pepper gene.
Characterization and expression profile of CaNAC2 pepper gene.
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DOI：
10.3389/fpls.2015.00755
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发表时间：
2015
影响因子：
5.6
通讯作者：
Zhang YY
中科院分区：
文献类型：
--
作者：
Guo WL;Wang SB;Chen RG;Chen BH;Du XH;Yin YX;Gong ZH;Zhang YY
植物特异性NAC （NAM、ATAF和CUC）转录因子在植物发育和胁迫调控中发挥着不同的作用。从aba调控的辣椒幼苗减法cDNA文库中鉴定出一个新的NAC蛋白转录物，该转录物与矮牵牛的namlike蛋白4同源。本文对该同源物（命名为CaNAC2）进行了鉴定，并研究了其在非生物胁迫耐受中的作用。结果表明，CaNAC2的n端结构域存在一个具有植物特异性和保守性的NAC结构域，该结构域编码410个氨基酸的多肽。系统发育分析表明，CaNAC2属于同源类群4d的NAC2亚群。CaNAC2蛋白亚细胞定位于酵母细胞核，在酵母细胞中具有转录活性。CaNAC2主要在种子和根中表达。冷、盐和ABA处理能强烈诱导CaNAC2的转录表达，而渗透胁迫和SA处理能抑制CaNAC2的转录表达。在病毒诱导的基因沉默辣椒幼苗中，CaNAC2基因的沉默增加了辣椒对冷胁迫的敏感性，延缓了盐诱导的叶片叶绿素降解。这些结果表明，CaNAC2基因可能参与了辣椒对非生物胁迫的抗性反应。
The plant-specific NAC (NAM, ATAF, and CUC) transcription factors have diverse role in development and stress regulation. A new transcript encoding NAC protein, homologous to nam-like protein 4 from Petunia was identified from an ABA-regulated subtractive cDNA library of Capsicum annuum seedling. Here, this homolog (named CaNAC2) from C. annuum was characterized and investigated its role in abiotic stress tolerance. Our results indicated that a plant-specific and conserved NAC domain was located in the N-terminus domain of CaNAC2 which was predicted to encode a polypeptide of 410 amino acids. Phylogenetic analysis showed that CaNAC2 belonged to the NAC2 subgroup of the orthologous group 4d. The protein CaNAC2 was subcellularly localized in the nucleus and it had transcriptional activity in yeast cell. CaNAC2 was expressed mainly in seed and root. The transcription expression of CaNAC2 was strongly induced by cold, salt and ABA treatment and inhibited by osmotic stress and SA treatment. Silence of CaNAC2 in virus-induced gene silenced pepper seedlings resulted in the increased susceptibility to cold stress and delayed the salt-induced leaf chlorophyll degradation. These results indicated that this novel CaNAC2 gene might be involved in pepper response to abiotic stress tolerance.