Humanoid robots to mechanically stress human cells grown in soft bioreactors
Humanoid robots to mechanically stress human cells grown in soft bioreactors
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DOI：
10.1038/s44172-022-00004-9
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发表时间：
2022-05
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影响因子：
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通讯作者：
P. Mouthuy;Sarah J B Snelling;Rafael Hostettler;Alona Kharchenko;Sarah Salmon;Alan Wainman;J. Mimpen;C. Paul;A. Carr
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文献类型：
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作者：
P. Mouthuy;Sarah J B Snelling;Rafael Hostettler;Alona Kharchenko;Sarah Salmon;Alan Wainman;J. Mimpen;C. Paul;A. Carr
20多年来，机器人生物反应器系统利用机械刺激促进了组织工程结构的生长。然而，我们仍然无法生产出可以转化为临床使用的功能性移植物。类人机器人提供了为移植物和植入物提供与生理相关的机械刺激的前景，这可能会加快它们的临床部署。为了研究仿人生物反应器的可行性，我们设计了一种可连接到改良型肌肉骨骼(MSK)仿人机器人肩关节的柔性生物反应器。我们证明成纤维细胞可以在这个小室中生长，同时在机械臂上进行生理性内收-外展。14天后对细胞转录组的初步评估表明，加载制度对基因表达谱有明显的影响。这些早期结果将有助于探索MSK类人机器人作为组织工程和生物材料测试应用的生物力学更现实的平台。
For more than 20 years, robotic bioreactor systems have facilitated the growth of tissue-engineered constructs using mechanical stimulation. However, we are still unable to produce functional grafts that can translate into clinical use. Humanoid robots offer the prospect of providing physiologically-relevant mechanical stimulation to grafts and implants which may expedite their clinical deployment. To investigate the feasibility of a humanoid bioreactor, we have designed a flexible bioreactor chamber that can be attached to a modified musculoskeletal (MSK) humanoid robot shoulder joint. We demonstrate that fibroblast cells can be grown in this chamber while undergoing physiological adduction-abduction on the robotic arm. A preliminary evaluation of the transcriptome of the cells after 14 days indicated a clear influence of the loading regime on the gene expression profile. These early results will facilitate the exploration of MSK humanoid robots as a biomechanically more realistic platform for tissue engineering and biomaterial testing applications.