Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense
Structure and bioactivity of the polysaccharides in medicinal plant Dendrobium huoshanense
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DOI：
10.1016/j.bmc.2008.04.042
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发表时间：
2008-06-01
影响因子：
3.5
通讯作者：
Wong, Chi-Huey
中科院分区：
文献类型：
--
作者：
Hsieh, Yves S. -Y.;Chien, Cheng;Wong, Chi-Huey
采用色谱、光谱、化学和酶法等多种技术对霍山石斛（Dendrobium hooshanense）的叶、茎细胞壁和粘液中提取的活性多糖进行了详细的结构测定。粘液多糖在活化鼠脾细胞以产生几种细胞因子（包括IFN-γ、IL-10、IL-6和IL-1 α）以及造血生长因子GM-CSF和G-CSF中表现出特异性功能。然而，从碱处理获得的脱乙酰粘液不能诱导细胞因子的产生。通过进一步的分级分离，证实了粘液组分的结构和生物活性。本研究首次为D.霍山蕨。(C)2008爱思唯尔有限公司版权所有。
Detailed structures of the active polysaccharides extracted from the leaf and stem cell walls and mucilage of Dendrobium huoshanense are determined by using various techniques, including chromatographic, spectroscopic, chemical, and enzymatic methods. The mucilage polysaccharide exhibits specific functions in activating murine splenocytes to produce several cytokines including IFN-gamma, IL-10, IL-6, and IL-1 alpha, as well as hematopoietic growth factors GM-CSF and G-CSF. However, the deacetylated mucilage obtained from an alkaline treatment fails to induce cytokine production. The structure and bioactivity of mucilage components are validated by further fractionation. This is the first study that provides clear evidence for the structure and activity relationship of the polysaccharide in D. huoshanense. (C) 2008 Elsevier Ltd. All rights reserved.