Overexpression of phenylalanine ammonia-lyase improves flavones production in transgenic hairy root cultures of Scutellaria baicalensis
Overexpression of phenylalanine ammonia-lyase improves flavones production in transgenic hairy root cultures of Scutellaria baicalensis
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DOI：
10.1016/j.procbio.2012.09.026
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发表时间：
2012-12-01
影响因子：
4.4
通讯作者：
Park, Sang Un
中科院分区：
文献类型：
--
作者：
Park, Nam Il;Xu, Hui;Park, Sang Un
利用发根农杆菌介导的转化体系，从黄芩中获得了苯丙氨酸解氨酶（PAL）的转基因毛状根系SbPAL 1、SbPAL 2和SbAPL 3。通过实时PCR证实了SbPAL基因的稳定遗传转化。这些转基因毛状根系产生更高量的黄酮（即，黄芩苷、黄芩素和汉黄芩素）比对照毛状根线。特别地，在SbPAL 1-、SbPAL 2-和SbPAL 3-过表达的毛状根中汉黄芩素含量比S.黄芩野生型根。本研究揭示了PAL在黄酮合成中的重要作用，证明了代谢工程在S.黄芩毛状根(C)2012爱思唯尔有限公司版权所有。
Transgenic hairy root lines from Scutellaria baicalensis overexpressing phenylalanine ammonia-lyase (PAL) (SbPAL1, SbPAL2, and SbAPL3) were established using an Agrobacterium rhizogenes-mediated transformation system. Stable genetic transformation with the SbPAL genes was confirmed by real-time PCR. These transgenic hairy root lines produced higher quantities flavones (i.e., baicalin, baicalein, and wogonin) than the control hairy root line. In particular, the wogonin content was increased by 4-11 times in the SbPAL1-, SbPAL2-, and SbPAL3-overexpressing hairy roots than the S. baicalensis wild-type roots. This research showed the importance of PAL in flavone biosynthesis and demonstrated the efficiency of metabolic engineering in S. baicalensis hairy roots. (C) 2012 Elsevier Ltd. All rights reserved.