The outer membrane protein, antigen 43, mediates cell-to-cell interactions within Escherichia coli biofilms
The outer membrane protein, antigen 43, mediates cell-to-cell interactions within Escherichia coli biofilms
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DOI：
10.1046/j.1365-2958.2000.02008.x
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发表时间：
2000-07-01
影响因子：
3.6
通讯作者：
Kolter, R
中科院分区：
文献类型：
--
作者：
Danese, PN;Pratt, LA;Kolter, R
agn43位点编码大肠杆菌的一种外膜蛋白，其转录受OxyR - DNA结合蛋白和Dam甲基化酶以相位可变的方式调控。尽管其调控机制已被充分研究，但直到现在Ag43的功能仍然难以捉摸。先前的研究表明，Ag43介导某些大肠杆菌菌株在液体培养中的自身聚集。鉴于这种表型，我们研究了Ag43在生物膜形成中的作用。在此，我们报道Ag43有助于大肠杆菌在葡萄糖基本培养基中形成生物膜，但在Luria - Bertani肉汤中则无此作用。此外，我们表明在丰富和基本环境中，鞭毛介导的运动对于生物膜形成都是必需的。总之，我们的研究结果表明，大肠杆菌在不同生长条件下利用通用和特定的基因集来形成生物膜。
Transcription of the agn43 locus, which specifies an outer membrane protein of Escherichia coli, is regulated in a phase-variable fashion by the OxyR-DNA binding protein and Dam methylase. Despite its well-characterized regulation, the function of Ag43 has remained elusive until now. Previous studies indicated that Ag43 mediates autoaggregation of certain strains of E. coli in liquid culture. Given this phenotype, we examined the role of Ag43 in biofilm formation. Here, we report that Ag43 contributes to E. coli biofilm formation in glucose-minimal medium, but not in Luria-Bertani broth. In addition, we show that flagellar-mediated motility is required for biofilm formation in both rich and minimal environments. Altogether, our results suggest that E. coli uses both common and specific gene sets for the development of biofilms under various growth conditions.