Antibody reactivity to latent and lytic antigens to human herpesvirus-8 in longitudinally followed homosexual men
Antibody reactivity to latent and lytic antigens to human herpesvirus-8 in longitudinally followed homosexual men
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DOI：
10.1086/345866
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发表时间：
2003-01-01
影响因子：
6.4
通讯作者：
Goedert, JJ
中科院分区：
文献类型：
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作者：
Biggar, RJ;Engels, EA;Goedert, JJ
人类疱疹病毒-8（HHV-8）的测试通常不同意哪些人被感染。为了评估多年来HHV-8抗体的变化，对1982年至1999年期间参加前瞻性研究的245名同性恋男性进行了研究。每年采集的样本通过免疫荧光法对HHV-8潜伏核抗原进行评价，并通过ELISA法对HHV-8裂解抗原K8.1抗体进行评价。当呈阳性时，大多数受试者的样本在两种检测中均呈持续反应性。溶解性抗体和潜伏性抗体的滴度多年来都在增加，通常是平行的，并且在人类免疫缺陷病毒感染的男性中更高。滴度的增加和表位识别的扩大表明HHV-8正在进行低度复制。在确定无症状人群的患病率时，使用多种检测方法是很重要的。由于感染较晚期的人抗体滴度较高，因此更容易检测，这可能会扭曲我们对HHV-8流行病学的理解。
Tests for human herpesvirus-8 (HHV-8) often disagree on which individuals are infected. To evaluate HHV-8 antibody changes over many years, 245 homosexual men enrolled in a prospective study between 1982 and 1999 were studied. Samples collected annually were evaluated by immunofluorescence to HHV-8 latent nuclear antigen and by an ELISA for antibody against the HHV-8 lytic antigen K8.1. When positive, samples from most subjects were persistently reactive in both assays. Titers to both lytic and latent antibodies increased for many years, usually in parallel, and were higher in human immunodeficiency virus-infected men. The increasing titers and expanding epitope recognition suggest ongoing, low-grade replication of HHV-8. Use of multiple assays is important in determining prevalence in asymptomatic populations. Because persons with more advanced infections have higher antibody titers, they are easier to detect, which may skew our understanding of the epidemiology of HHV-8.