Dustborne microorganisms in the atmosphere over an Asian dust source region, Dunhuang
Dustborne microorganisms in the atmosphere over an Asian dust source region, Dunhuang
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DOI：
10.1007/s11869-008-0024-9
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发表时间：
2008-12-01
影响因子：
5.1
通讯作者：
Iwasaka, Yasunobu
中科院分区：
文献类型：
--
作者：
Kakikawa, Makiko;Kobayashi, Fumihisa;Iwasaka, Yasunobu
沙尘事件将微生物注入大气，并可能促进生物颗粒的扩散，影响背风生态系统和人类健康。本研究采用非培养法对亚洲沙尘源塔克拉玛干沙漠大气中的尘载微生物进行了鉴定。在亚洲沙尘源区敦煌，用气球在距地面800 m处收集沙尘。直接从过滤器收集的粉尘中提取DNA后，对微生物的16S和18S rRNA基因进行扩增、克隆和测序。rDNA序列数据表明，灰尘颗粒包括真菌密切相关的Rickenella腓骨，Ceriporiopsis gilvescens，和属于短芽孢杆菌属，葡萄球菌属，红球菌属，Delftia，假单胞菌属，和根癌农杆菌属的细菌。这些结果表明，敦煌地区大气中的尘埃颗粒可能携带了大量的真菌和细菌，并可能在背风生态系统中发挥重要作用。
The dust event injects microorganisms into the atmosphere and could facilitate the dispersal of biological particles affecting leeward ecosystem and human health. In this study, the dustborne microorganisms in the atmosphere over the Taklimakan Desert, Asian dust source, were identified by culture-independent method. Dusts were collected using a balloon at about 800 m above the ground in an Asian dust source region, Dunhuang. After DNA were directly extracted from the dusts collected filters, 16S and 18S rRNA genes of microorganisms were amplified, cloned, and sequenced. The rDNA sequence data indicated that dust particles include fungi closely related to Rickenella fibula, Ceriporiopsis gilvescens, and bacteria belonging to the genus Brevibacillus, Staphylococcus, Rhodococcus, Delftia, Pseudomonas, and Agrobacterium tumefaciens. These results suggest that dust particles in the atmosphere over Dunhuang could carry these many fungi and bacteria and might play a significant role in leeward ecosystem.