Uptake of noradrenaline by adrenergic nerves, smooth muscle and connective tissue in isolated perfused arteries and its correlation with the vasoconstrictor response.
Uptake of noradrenaline by adrenergic nerves, smooth muscle and connective tissue in isolated perfused arteries and its correlation with the vasoconstrictor response.
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分离灌注动脉中肾上腺素能神经、平滑肌和结缔组织对去甲肾上腺素的摄取及其与血管收缩反应的相关性。
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发表时间：
1968
期刊：
影响因子：
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通讯作者：
J. Gillespie
中科院分区：
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作者：
O. V. Avakian;J. Gillespie
用含有大量去甲肾上腺素的血液灌注猫脾，当暴露于甲醛蒸汽时，会导致平滑肌细胞产生这种儿茶酚胺的荧光特征（吉莱斯皮和汉密尔顿，1967）。荧光的发展被生化测定的去甲肾上腺素的保留所抑制（吉莱斯皮，汉密尔顿和Hosie，1967）。平滑肌细胞在保留去甲肾上腺素的程度和保留部位上均不同;在小梁和囊平滑肌中，荧光轻微并局限于细胞的外周，而在动脉平滑肌中，荧光强烈并存在于细胞内。在灌注的脾脏中，很难确定所有平滑肌细胞都同等地暴露于去甲肾上腺素，在本实验中，用盐水灌注一段孤立的动脉，希望能尽量减少这种困难，以便确定去甲肾上腺素浓度与荧光程度之间的关系。在脾脏实验中，α-受体阻断剂酚苄明阻止了动脉平滑肌荧光的发展（吉莱斯皮和汉密尔顿，1966），表明α-受体可能参与了摄取。如果是这样的话，可以预期酚苄明会以平行的方式干扰荧光的产生和响应。血管收缩反应很容易在灌注动脉中测量，因此非常适合这种相关性。对其中一些结果的简要介绍以前曾发表过（Avakian &吉莱斯皮，1967）。
Perfusion of the cat spleen with blood containing large quantities of noradrenaline causes the smooth muscle cells, when exposed to formaldehyde vapour, to develop the fluorescence characteristic of this catecholamine (Gillespie & Hamilton, 1967). The development of fluorescence is paralleled by the retention of noradrenaline assayed biochemically (Gillespie, Hamilton & Hosie, 1967). The smooth muscle cells differed both in the degree to which they retained noradrenaline and in the site of retention; in trabecular and capsular smooth muscle the fluorescence was slight and confined to the periphery of the cells, whereas in the smooth muscle of arteries the fluorescence was intense and present within the cells. In the perfused spleen it was difficult to be sure that all smooth muscle cells were equally exposed to noradrenaline and in the present experiments with an isolated length of artery perfused with saline it was hoped to minimize this difficulty so that the relationship between the concentration of noradrenaline and the degree of fluorescence could be determined. In the experiments on the spleen, the a-receptor blocking agent phenoxybenzamine prevented the development of fluorescence of arterial smooth muscle (Gillespie & Hamilton, 1966), suggesting that a-receptors might be involved in the uptake. If this were so, one would expect phenoxybenzamine to interfere with the development of fluorescence and with the response in a parallel fashion. The vasoconstrictor response is easily measured in the perfused artery and it is therefore very suitable for this correlation. A brief account of some of these results has previously been published (Avakian & Gillespie, 1967).