A novel rat model of temporomandibular disorder with improved face and construct validities.
A novel rat model of temporomandibular disorder with improved face and construct validities.
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DOI：
10.1016/j.lfs.2021.120023
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发表时间：
2021-12-01
期刊：
影响因子：
6.1
通讯作者：
Taylor NE
中科院分区：
文献类型：
--
作者：
Phero A;Ferrari LF;Taylor NE
颞下颌关节疾病是一组以颞下颌关节（TMJ）及其周围肌肉/组织疼痛为特征的口腔面部疾病。疼痛性颞下颌关节功能障碍（TMD）动物模型是研究症状性颞下颌关节及相关结构障碍机制的重要工具。我们测试了一个假设，即需要一个诱发因素和一个促成因素才能在大鼠身上产生疼痛的TMD，使用ratgnawmeter（一种根据大鼠咀嚼两个障碍物所需的时间来确定颞下颌疼痛的设备）。咀嚼时间增加与TMJ注射福尔马林产生的伤害性行为相关（2.5%），证实咀嚼时间是疼痛性TMD的指标。大鼠只暴露于易感因素，卡拉胶诱导的TMJ炎症或or -486持续抑制儿茶酚- o -甲基转移酶（COMT）酶，咀嚼时间没有变化。然而，当与突发事件相结合时，即连续7天每天1小时的下颌伸展所产生的夸张的张口，产生了强大的功能障碍。这些结果验证了ratgnawmeter是通过评估咀嚼时间来评估功能性TMD疼痛的有效方法，并且该方案作为具有面部和结构效度的模型来研究症状性TMD机制。这项研究表明，必须存在一个易感因素，以便对颞下颌系统的侮辱产生痛苦的功能障碍。这些因素的综合作用可能解释了为什么并非所有经历创伤性事件的患者，如张口过大，都会发展为颞下颌颞痛。
Temporomandibular disorders are a cluster of orofacial conditions that are characterized by pain in the temporomandibular joint (TMJ) and surrounding muscles/tissues. Animal models of painful temporomandibular dysfunction (TMD) are valuable tools to investigate the mechanisms responsible for symptomatic temporomandibular joint and associated structures disorders. We tested the hypothesis that a predisposing and a precipitating factor are required to produce painful TMD in rats, using the ratgnawmeter, a device that determines temporomandibular pain based on the time taken for the rat to chew through two obstacles. Increased time in the ratgnawmeter correlated with nociceptive behaviors produced by TMJ injection of formalin (2.5%), confirming chewing time as an index of painful TMD. Rats exposed only to predisposing factors, carrageenan-induced TMJ inflammation or sustained inhibition of the catechol-O-methyltransferase (COMT) enzyme by OR-486, showed no changes in chewing time. However, when combined with a precipitating event, i.e., exaggerated mouth opening produced by daily 1-hour jaw extension for 7 consecutive days, robust function impairment was produced. These results validate the ratgnawmeter as an efficient method to evaluate functional TMD pain by evaluating chewing time, and this protocol as a model with face and construct validities to investigate symptomatic TMD mechanisms. This study suggests that a predisposition factor must be present in order for an insult to the temporomandibular system to produce painful dysfunction. The need for a combined contribution of these factors might explain why not all patients experiencing traumatic events, such as exaggerated mouth opening, develop TMDs.
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影响因子：
3.5
作者：
Diatchenko, L;Slade, GD;Maixner, W
通讯作者：
Maixner, W
影响因子：
1.9
作者：
Chang, H;Israel, H
通讯作者：
Israel, H
DOI：
10.14219/jada.archive.1990.0043
发表时间：
1990-03-01
影响因子：
3.9
作者：
DWORKIN, SF;HUGGINS, KH;SOMMERS, E
通讯作者：
SOMMERS, E
DOI：
10.15386/cjmed-485
发表时间：
2015
期刊：
Clujul medical (1957)
影响因子：
--
作者：
Chisnoiu AM;Picos AM;Popa S;Chisnoiu PD;Lascu L;Picos A;Chisnoiu R
通讯作者：
Chisnoiu R
影响因子：
7.3
作者：
Ferreira, SH;Cunha, FQ;Poole, S
通讯作者：
Poole, S