PPARγ stimulation promotes neurite outgrowth in SH-SY5Y human neuroblastoma cells
PPARγ stimulation promotes neurite outgrowth in SH-SY5Y human neuroblastoma cells
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DOI：
10.1016/j.neulet.2009.03.014
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发表时间：
2009-04-24
影响因子：
2.5
通讯作者：
Fantozzi, Roberto
中科院分区：
文献类型：
--
作者：
Miglio, Gianluca;Rattazzi, Lorenza;Fantozzi, Roberto
一些证据表明，PPAR γ刺激促进神经元分化。然而，迄今为止，没有数据描述的影响，过氧化物酶体增殖物激活受体γ激动剂对神经突生长。在这里，我们评估了吡格列酮，一种合成的过氧化物酶体增殖物激活受体γ激动剂，对SH-SY 5 Y人神经母细胞瘤细胞分化和神经突生长的影响。我们的研究结果表明，吡格列酮以浓度依赖性方式促进细胞分化和细胞突起的生长，在100 nM-1 μ M时效果最大。它显著增加了平均突起长度和神经突承载细胞的百分比。此外，这些作用伴随着p42和p44丝裂原活化蛋白激酶的显著活化。总之，尽管是初步的，这些研究结果表明，PPAR γ刺激可能有助于神经元网络内适当的神经元连接的发展和维持的可能性。(C)2009爱思唯尔爱尔兰有限公司保留所有权利。
Several evidences indicate that PPAR gamma stimulation promotes neuronal differentiation. However, to date, no data describe the effects of PPAR gamma agonists on neurite outgrowth. Here we have evaluated the effects of pioglitazone, a synthetic PPAR gamma agonist, on differentiation and neurite outgrowth in SH-SY5Y human neuroblastoma cells. Our results show that pioglitazone promotes cell differentiation and the outgrowth of cell processes in a concentration-dependent manner with the maximal effect at 100 nM-1 mu M. It significantly increases both the mean process length and the percentage of neurite-bearing cells. In addition, these effects are accompanied by significant activation of p42 and p44 mitogen-activated protein kinases. In conclusion, albeit preliminary, these findings suggest the possibility that PPAR gamma stimulation may contribute to the development and maintenance of a proper neuronal connectivity within neuronal networks. (C) 2009 Elsevier Ireland Ltd. All rights reserved.