Clavukoellians G-K, New Nardosinane and Aristolane Sesquiterpenoids with Angiogenesis Promoting Activity from the Marine Soft Coral Lemnalia sp.
Clavukoellians G-K, New Nardosinane and Aristolane Sesquiterpenoids with Angiogenesis Promoting Activity from the Marine Soft Coral Lemnalia sp.
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来自海洋软珊瑚 Lemnalia sp. 的 Clavukoellians G-K、新 Nardosinane 和 Aristolane 倍半萜类化合物具有促进血管生成的活性。
DOI：
10.3390/md18030171
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发表时间：
2020-03-01
期刊：
影响因子：
5.4
通讯作者：
Li, Guoqiang
中科院分区：
文献类型：
--
作者：
Wang, Qi;Tang, Xuli;Li, Guoqiang
对南海西沙群岛采集的海洋软珊瑚Lemnalia sp.进行化学鉴定，分离到4个新的nardosinane型倍半萜，即clavukoellians G-J（1-4）和1个新的马托马烷倍半萜，即clavukoellian K(5)，以及5个已知化合物，6-10。分离得到的天然产物的结构是基于HRESIMS和NMR等多种光谱技术进行的，而它们的绝对构型是通过ECD光谱与计算ECD数据的比较来确定的。在转基因荧光斑马鱼（Tg(vegfr2:GFP)）模型中，对分离得到的新化合物1 ~ 5进行了抗血管生成和促血管生成活性评价。定量分析显示，化合物5在ptk787诱导的斑马鱼血管损伤模型2.5 μ m下具有促血管生成活性。数据显示，化合物5显著促进血管生成，且呈剂量依赖性。
The chemical examination of the marine soft coral Lemnalia sp., collected at the Xisha islands in the South China Sea, resulted in the isolation of four new nardosinane-type sesquiterpenoids, namely clavukoellians G-J (1-4), and one new aristolane sesquiterpene, namely clavukoellian K (5), together with five known compounds, 6-10. The structure elucidation of the isolated natural products was based on various spectroscopic techniques including HRESIMS and NMR, while their absolute configurations were resolved on the basis of comparisons of the ECD spectra with the calculated ECD data. The isolated new compounds 1-5 were evaluated for their anti- and pro- angiogenesis activities in a transgenic fluorescent zebrafish (Tg(vegfr2:GFP)) model. Quantitative analysis revealed that compound 5 displayed pro-angiogenesis activity in a PTK787-induced vascular injury zebrafish model at 2.5 mu M. Data showed that compound 5 significantly promoted the angiogenesis in a dose-dependent manner.