Mitochondria are impaired in the adipocytes of type 2 diabetic mice
Mitochondria are impaired in the adipocytes of type 2 diabetic mice
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DOI：
10.1007/s00125-006-0170-2
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发表时间：
2006-04-01
期刊：
影响因子：
8.2
通讯作者：
Ko, YG
中科院分区：
文献类型：
--
作者：
Choo, HJ;Kim, JH;Ko, YG
目的/假设：本研究的目的是确认线粒体功能障碍与2型糖尿病之间的联系。材料和方法：采用MitoTracker染色法和电镜观察12周龄雄性野生型、肥胖型（ob/ob）和糖尿病型（db/db）小鼠白色脂肪组织中线粒体蛋白水平、线粒体DNA含量、线粒体功能和形态。结果：在研究的所有小鼠的肝脏和肌肉中发现线粒体蛋白的水平非常相似。然而，db/db小鼠的脂肪细胞水平大大降低，而野生型和ob/ob小鼠则没有。在db/db小鼠的脂肪细胞中，线粒体DNA水平也显著降低。MitoTracker染色和电镜下显示db/db小鼠脂肪细胞线粒体数量减少。呼吸和脂肪酸氧化研究表明db/db小鼠脂肪细胞线粒体功能障碍。有趣的是，使用罗格列酮（一种增强胰岛素敏感性的药物）治疗的db/db小鼠的脂肪细胞中线粒体和线粒体蛋白的产生有所增加。结论/解释：综上所述，这些数据表明，脂肪组织中的线粒体丢失与2型糖尿病的发生有关。
Aims/hypothesis: The aim of this study was to confirm a link between mitochondrial dysfunction and type 2 diabetes. Materials and methods: Cellular levels of mitochondrial proteins, cellular mitochondrial DNA content, and mitochondrial function and morphology were assessed by MitoTracker staining and electron microscopy, in white adipose tissue of 12-week-old male wild-type, obese (ob/ob), and diabetic (db/db) mice. Results: Levels of mitochondrial proteins were found to be very similar in the livers and muscles of all the mice studied. However, levels were greatly decreased in the adipocytes of db/db mice, but not in those of the wild-type and ob/ob mice. Levels of mitochondrial DNA were also found to be considerably reduced in the adipocytes of db/db mice. MitoTracker staining and under electron microscopy revealed that the number of mitochondria was reduced in adipocytes of db/db mice. Respiration and fatty acid oxidation studies indicated mitochondrial dysfunction in adipocytes of db/db mice. Interestingly, there was an increase in mitochondria and mitochondrial protein production in adipocytes of db/db mice treated with rosiglitazone, an agent that enhances insulin sensitivity. Conclusions/interpretation: Taken together, these data indicate that mitochondrial loss in adipose tissue is correlated with the development of type 2 diabetes.