Coupling thin-layer chromatography with vibrational cooling matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for the analysis of ganglioside mixtures
Coupling thin-layer chromatography with vibrational cooling matrix-assisted laser desorption/ionization Fourier transform mass spectrometry for the analysis of ganglioside mixtures
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DOI：
10.1021/ac0491556
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发表时间：
2004-11-01
影响因子：
7.4
通讯作者：
Costello, CE
中科院分区：
文献类型：
--
作者：
Ivleva, VB;Elkin, YN;Costello, CE
薄层色谱法（TLC），这是广泛用于糖脂，寡糖，脂质，以及环境和药物利益的化合物的分离，可以很容易地耦合到基质辅助激光解吸/电离（MALDI）飞行时间质谱仪，但这种安排通常妥协质谱分辨率由于TLC表面的不规则性。然而，TLC可以耦合到外部离子源MALDI-Fourier变换（FT）MS仪器，而不会影响质谱的质量准确度和分辨率。此外，当FTMS具有振动冷却的MALDI离子源时，易碎的糖脂可以从TLC板上解吸附而不碎裂，甚至可以从“热”基质（如α-氰基-4-羟基肉桂酸）上解吸附完整分子。在这项工作中，使用TLC分离全脑神经节苷脂; TLC板直接连接到MALDI靶标，其中神经节苷脂被解吸、电离，并在FTMS中以> 70 000的分辨率检测。
Thin-layer chromatography (TLC), which is widely used for separation of glycolipids, oligosaccharides, lipids, and compounds of environmental and pharmaceutical interest, can be readily coupled to matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometers, but this arrangement usually compromises mass spectral resolution due to the irregularity of the TLC surface. However, TLC can be coupled to an external ion source MALDI-Fourier transform (FT) MS instrument without compromising mass accuracy and resolution of the spectra. Furthermore, when the FTMS has a vibrationally cooled MALDI ion source, fragile glycolipids can be desorbed from TLC plates without fragmentation, even to the point that desorption of intact molecules from "hot" matrixes such as alpha-cyano-4-hydroxycinnamic acid is possible. In this work, whole brain gangliosides are separated using TLC; the TLC plates are attached directly to the MALDI target, where the gangliosides are desorbed, ionized, and detected in the FTMS with > 70 000 resolving power.