Loss of the retinoblastoma susceptibility gene (RB1) is a frequent and early event in prostatic tumorigenesis.
Loss of the retinoblastoma susceptibility gene (RB1) is a frequent and early event in prostatic tumorigenesis.
复制标题
视网膜母细胞瘤易感基因（RB1）的丧失是前列腺肿瘤发生的常见和早期事件。
DOI：
10.1038/bjc.1994.482
复制
发表时间：
1994-12
影响因子：
8.8
通讯作者：
Neoptolemos, J P
中科院分区：
文献类型：
--
作者：
Phillips, S M;Barton, C M;Lee, S J;Morton, D G;Wallace, D M;Lemoine, N R;Neoptolemos, J P
RB 1基因的丢失是许多肿瘤发生和发展的重要事件。对43例前列腺癌和10例良性前列腺肥大（BPH）患者的前列腺组织进行了RB 1基因杂合性缺失的研究。用两种技术研究了4个基因内多态位点。这就是限制性片段长度多态性（RFLP）。Southern印迹和与p123m1.8和p68RS2.0探针的杂交（分别针对内含子1和17）以及聚合酶链反应（PCR）以扩增内含子17和20内的基因座。使用NCL-RB抗体通过免疫组织化学测定9例癌症患者和4例BPH患者的蛋白产物（pRB）表达。在40例有信息的癌症患者中，24例（60%）发现杂合性丢失。在早期和低级别癌症中，RB 1丢失的频率与更晚期的癌症相似。1例BPH患者RB 1缺失。pRB的表达在7种癌症中完全不存在，在另外2种癌症中显著减少，而核pRB染色总是存在于BPH区域中，无论是与含癌组织一起还是单独与BPH一起。我们的结论是RB 1的损失是前列腺肿瘤发生的早期事件。
Loss of the RB1 gene is an important event in the initiation and progression of many tumours. Prostate tissue from 43 patients with prostate cancers and ten with benign prostatic hypertrophy (BPH) were studied for loss of heterozygosity of the RB1 gene. Four intragenic polymorphic loci were studied with two techniques. These were restriction fragment length polymorphism (RFLP). Southern blotting and hybridisation with the p123m1.8 and p68RS2.0 probes (to introns 1 and 17 respectively) and also the polymerase chain reaction (PCR) to amplify loci within introns 17 and 20. Protein product (pRB) expression was determined by immunohistochemistry using the NCL-RB antibody in nine patients with cancer and four patients with BPH. Loss of heterozygosity was found in 24 out of 40 (60%) informative patients with cancer. Loss of RB1 occurred with a similar frequency in early-stage and low-grade cancers as in more advanced cancers. Loss of RB1 was also found in one patient with BPH. Expression of pRB was completely absent from seven cancers and markedly reduced in the other two, while nuclear pRB staining was always present in areas of BPH, whether alongside cancer-containing tissue or with BPH alone. We conclude that loss of RB1 is an early event in prostatic tumorigenesis.