Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer.
Bone morphogenetic protein encapsulated with a biodegradable and biocompatible polymer.
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用可生物降解和生物相容性聚合物封装的骨形态发生蛋白。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
N. Nakabayashi
中科院分区：
文献类型：
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作者：
M. Isobe;M. Isobe;T. Amagasa;S. Oida;Y. Yamazaki;K. Ishihara;N. Nakabayashi
为了开发骨形态发生蛋白（BMP）的控释系统，使用水包油包水乳液通过界面沉淀法制备了含有BMP的聚（DL-丙交酯-共-乙交酯）（PLGA）胶囊。检查了 PLGA 胶囊的表面形貌、粒径分布和水解降解率。使用异硫氰酸荧光素标记的 BMP 测定 BMP 的体外包封率和释放率。 PLGA 胶囊释放的 BMP 量在第 3 天至第 5 天之间增加。此外，还通过大鼠皮下植入检查了封装在 PLGA 中的 BMP 诱导体内骨形成的有效性。植入后 3 周，通过对收获的组织进行组织学检查来确定胶囊的完全消化和包括骨髓在内的新骨形成。这些结果表明，用 PLGA 封装 BMP 可能是一种有前途的临床诱导骨方法。
To develop a controlled release system for bone morphogenetic protein (BMP), poly(DL-lactide-co-glycolide) (PLGA) capsules containing BMP were prepared by an interfacial precipitation method using a water-in-oil-in-water emulsion. The surface morphology, particle size distribution, and hydrolytic degradation rate of the PLGA capsules were examined. The encapsulation yield and release rate of BMP in vitro were measured using fluorescein isothiocyanate-labeled BMP. The amount of BMP released from PLGA capsules increased between days 3 and 5. In addition, the effectiveness of BMP encapsulated in PLGA to induce bone formation in vivo was also examined by subcutaneous implantation in rats. Complete digestion of the capsules and new bone formation including bone marrow were identified by histologic examination of harvested tissues at 3 weeks after implantation. These results demonstrated that encapsulation of BMP with PLGA could be a promising method to induce bone in clinics.