A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii.
A self-splicing intron in the small subunit rRNA gene of Pneumocystis carinii.
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卡氏肺囊虫小亚基 rRNA 基因中的自剪接内含子。
DOI：
10.1093/nar/17.13.5349
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发表时间：
1989
影响因子：
14.9
通讯作者：
Edman,JC
中科院分区：
文献类型：
--
作者：
Sogin,ML;Edman,JC
卡氏肺孢子虫小亚基rRNA（16S−like rRNA）的编码区含有390个额外的碱基对，而其他真核生物的rRNA基因高度保守。该序列具有I组自我剪接内含子的所有特征，并中断了16S−样rRNA 3'末端附近的遗传保守螺旋。在体外转录含有推定的组I内含子能够进行“自我剪接”的反应，这需要鸟嘌呤核苷酸的存在。
The coding region for the Pneumocyststis carinii small subunit rRNA (16S−like rRNA) contains 390 extra ba3e pairs in a region that is highly conservedin other eukaryotic rRNA genes. The sequence has all the elementscharacteristic of group I self-splicing introns and interrupts thephylogenetically conserved helix proximal to the 3' terminus of the 16S−like rRNA. In vitro transcripts containing the putative group I intronwere capable of undergoing a “self-splicing“ reaction which required the presenceof guanine nucleotide.