WBC reduction reduces storage-associated RBC adhesion to human vascular endothelial cells under conditions of continuous flow in vitro
WBC reduction reduces storage-associated RBC adhesion to human vascular endothelial cells under conditions of continuous flow in vitro
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DOI：
10.1046/j.1537-2995.2003.00310.x
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发表时间：
2003-02-01
期刊：
影响因子：
2.9
通讯作者：
Chin-Yee, IH
中科院分区：
文献类型：
--
作者：
Luk, CS;Gray-Statchuk, LA;Chin-Yee, IH
背景技术背景：储存时间，白细胞减少，照射对红细胞粘附血管内皮细胞的影响是未知的，并在连续flow.Study设计和方法的条件下进行了研究：收集了32个红细胞单位，分为三组，非白细胞减少（NWR），白细胞减少（WR），和照射白细胞减少。在储存第1、15和28天取出RBC等分试样进行分析。然后在连续流过人脐静脉内皮细胞单层的条件下，将RBC悬液以1.5%Hct灌注在蛋白质贫乏的培养基中。在每个载玻片上，在10分钟内对25个随机选择的位点进行录像，并对粘附于内皮细胞单层的RBC的数量进行计数。结果：与第1天和第15天相比，储存28天的NWR RBC表现出更大程度的粘附于内皮细胞（p < 0.03）。在第28天，WR组具有比NWR组显著更少的粘附RBC（p < 0.01）。结论：NWR红细胞长期保存可增加红细胞与内皮细胞的粘附。储存前WBC减少消除了储存持续时间对粘附增加的影响。需要进行研究以评估是否发生体内效应。
BACKGROUND: The effects of storage duration, WBC reduction, and irradiation on RBC adherence to vascular endothelia are unknown and are investigated under conditions of continuous flow.STUDY DESIGN AND METHODS: Thirty-two RBC units were collected and divided into three groups, non-WBC-reduced (NWR), WBC-reduced (WR), and irradiated-WBC-reduced. Aliquots of RBCs were removed on Days 1, 15, and 28 of storage for analysis. The RBC suspensions were then perfused at a 1.5 percent Hct in a protein-poor medium under conditions of continuous flow over human umbilical vein endothelial cell monolayers. On each slide, 25 randomly chosen sites were videorecorded over 10 minutes, and the number of RBCs adherent to the endothelial cell monolayer was counted.RESULTS: NWR RBCs stored for 28 days demonstrated a greater degree of adherence to endothelial cells compared to Days 1 and 15 (p < 0.03). The WR group had significantly fewer adherent RBCs than the NWR group on day 28 (p < 0.01). Irradiation had no effect on RBC adherence.CONCLUSION: Prolonged storage of NWR RBCs increases RBC adherence to endothelial cells in vitro. WBC reduction before storage abrogates the effect of storage duration on increased adhesion. Studies to assess whether an in vivo effect occurs are required.