The nicotinic acetylcholine receptor gene family of the malaria mosquito, Anopheles gambiae
The nicotinic acetylcholine receptor gene family of the malaria mosquito, Anopheles gambiae
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DOI：
10.1016/j.ygeno.2004.09.001
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发表时间：
2005-02-01
期刊：
影响因子：
4.4
通讯作者：
Sattelle, DB
中科院分区：
文献类型：
--
作者：
Jones, AK;Grauso, M;Sattelle, DB
烟碱型乙酰胆碱受体（nAChRs）介导昆虫神经系统中的快速胆碱能突触传递，并且是广泛销售的杀虫剂的靶标。我们已经确定了nAChR基因家族的基因组的疟疾蚊子的载体，冈比亚按蚊，是第二个完整的昆虫nAChR基因家族的黑腹果蝇。与果蝇一样，按蚊也有10个nAChR亚基，这两种昆虫之间存在明显的同源关系。有趣的是，D β 2和D β 3的按蚊直系同源物具有限定α亚基的邻近半胱氨酸。与Dalpha 4和Dalpha 6一样，按蚊直向同源物在等效外显子处选择性剪接。逆转录-聚合酶链反应分析表明，RNA A-至-1编辑位点之间的果蝇和α 7-2的烟草夜蛾，Heliothis virescens的Dalpha 6保守，不共享与按蚊的nAChR亚基的等效。事实上，在Dbeta 1，Dalpha 5和Dalpha 6的功能重要区域中鉴定的RNA编辑位点在蚊子直系同源物中不保守，表明在昆虫双翅目中靶向编辑的RNA分子存在相当大的差异。这些发现进一步阐明了nAChR亚基的多样性，并可能为通过增强我们对经验证的蚊子杀虫剂靶标的理解来开发改进的疟疾控制剂提供有用的基础。(C)2004年爱思唯尔公司All rights reserved.
Nicotinic acetylcholine receptors (nAChRs) mediate fast cholinergic synaptic transmission in the insect nervous system and are targets of widely selling insecticides. We have identified the nAChR gene family from the genome of the malaria mosquito vector, Anopheles gambiae, to be the second complete insect nAChR gene family described following that of Drosophila melanogaster. Like Drosophila, Anopheles possesses 10 nAChR subunits with orthologous relationships evident between the two insects. Interestingly, the Anopheles orthologues of Dbeta2 and Dbeta3 possess the vicinal cysteines that define alpha subunits. As with Dalpha4 and Dalpha6, the Anopheles orthologues are alternatively spliced at equivalent exons. Reverse transcription-polymerase chain reaction analysis shows that RNA A-to-1 editing sites conserved between Dalpha6 of Drosophila and alpha 7-2 of the tobacco budworm, Heliothis virescens, are not shared with the equivalent nAChR subunit of Anopheles. Indeed, RNA-editing sites identified in functionally significant regions of Dbeta1, Dalpha5, and Dalpha6 are not conserved in the mosquito orthologues, indicating considerable divergence of RNA molecules targeted for editing within the insect order Diptera. These findings shed further light on the diversity of nAChR subunits and may present a useful basis for the development of improved malaria control agents by enhancing our understanding of a validated mosquito insecticide target. (C) 2004 Elsevier Inc. All rights reserved.