Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina.
Apical polarization of N-CAM in retinal pigment epithelium is dependent on contact with the neural retina.
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DOI：
10.1083/jcb.121.2.335
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发表时间：
1993-04
影响因子：
7.8
通讯作者：
Rodriguez-Boulan, E
中科院分区：
文献类型：
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作者：
Gundersen, D;Powell, S K;Rodriguez-Boulan, E
视网膜色素上皮（RPE）在上皮细胞中是独特的，因为其顶面不面向管腔，而是专门用于与神经视网膜相互作用。参与RPE与神经视网膜相互作用的分子尚不清楚。我们在这里表明，神经细胞粘附分子（N-CAM）被发现在原位RPE的顶端表面和光感受器的外段，实现两个结构之间的粘附作用的重要条件。引人注目的是，RPE的培养导致N-CAM快速重新分布到基底外侧表面。这不是由于同种型转变，因为培养细胞表达的N-CAM（140 kD）与体内RPE表达的相同。相反，N-CAM的反向极性似乎是由于RPE与神经视网膜的光感受器之间的接触中断而导致的。我们认为，N-CAM在RPE和光感受器参与这些相互作用。
The retinal pigment epithelium (RPE) is unique among epithelia in that its apical surface does not face a lumen, but, instead, is specialized for interaction with the neural retina. The molecules involved in the interaction of the RPE with the neural retina are not known. We show here that the neural cell adhesion molecule (N-CAM) is found both on the apical surface of RPE in situ and on the outer segments of photoreceptors, fulfilling an important requisite for an adhesion role between both structures. Strikingly, culture of RPE results in rapid redistribution of N-CAM to the basolateral surface. This is not due to an isoform shift, since the N-CAM expressed by cultured cells (140 kD) is the same as that expressed by RPE in vivo. Rather, the reversed polarity of N-CAM appears to result from the disruption of the contact between the RPE and the photoreceptors of the neural retina. We suggest that N-CAM in RPE and photoreceptors participate in these interactions.