Spin trapping of free radical products of CCI4 activation using pulse radiolysis and high energy radiation procedures
Spin trapping of free radical products of CCI4 activation using pulse radiolysis and high energy radiation procedures
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使用脉冲辐射分解和高能辐射程序自旋捕获 CCI4 活化的自由基产物
DOI：
10.1016/0014-5793(80)80461-3
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发表时间：
1980
期刊：
影响因子：
3.5
通讯作者：
T. Slater
中科院分区：
文献类型：
--
作者：
A. Tomasi;E. Albano;K. Lott;T. Slater
自由基被认为是许多类型组织损伤中的活性中间体[1，2]。电子自旋谱（ESR）和脉冲辐解技术正越来越多地被用于鉴定所涉及的自由基，并获得有关其化学反应性的定量数据[3，4]。四氯化碳是一种重要且研究较多的肝毒素，通过NADPH细胞色素P450电子传递链代谢为通常被认为是三氯甲基自由基的活化中间体[5，6]。尝试[7-91]使用直接ESR分析来检测整个肝脏或肝脏部分中的CCl自由基尚未成功，因此注意力被引导到不太直接的ESR自旋捕获技术[10 -121]。尽管该方法在体外和体内研究中很有前景，但在复杂生物膜环境中产生的自由基物质的ESR光谱的解释中存在复杂性[12，131]。出于这个原因，我们研究了CCl的相互作用，与自旋陷阱苯基丁基硝酮在一个简化的模型系统中，CCl的自由基产生的脉冲的高能电子。我们之前已经获得了该模型系统中CCl及其过氧衍生物CClaO [14]反应性的动力学数据。这些数据，连同13 CC 14的ESR分析（如[1]中所建议的），使我们能够明确地鉴定CCl和CCl，O的自旋加合物;微粒体系统、完整肝细胞和体内条件下也报告了相同的光谱。
Free radicals have been suggested as activated intermediates in many types of tissue injury [1, 2]. The techniques of electron spin spectroscopy (ESR) and pulse radiolysis are being increasingly used to characterise the free radicals involved, and to obtain quantitative data on their chemical reactivity [3, 4]. Carbon tetrachloride is an important and much studied hepatoxin, which is metabolised by the NADPHcytochrome P450 electron-transport chain to an activated intermediate generally considered to be the trichloromethyl radical [5, 6]. Attempts [7-91 to detect the Ccl; free radical in whole liver or liver fractions using direct ESR analysis have not been successful, and attention has consequently been directed to the less direct ESR spin trapping techniques [lo-121. Although this procedure is promising for studies both in vitro and in vivo, there are complications in interpretation of the ESR spectra of the free radical species produced in complex biomembrane environments[12,131. For this reason, we have studied the interaction of Ccl; with the spin-trap phenylbutylnitrone in a simplified model system where the Ccl; radicals are produced by a pulse of high energy electrons. We have previously obtained kinetic data on the reactivity of Ccl; and its peroxy-derivative CClaO;[14] in this model system. These data, together with ESR analysis with 13CC14 (as suggested in [l]), allow us to unequivocally identify the spintrap adducts of Ccl; and CCl, O;; identical spectra are also reported for microsomal systems, intact hepatocytes and under conditions in vivo.