A disposable, simple, fast and low-cost paper-based biosensor and its application to the determination of glucose in commercial orange juices
A disposable, simple, fast and low-cost paper-based biosensor and its application to the determination of glucose in commercial orange juices
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DOI：
10.1016/j.foodchem.2018.05.082
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发表时间：
2018-11-01
期刊：
影响因子：
8.8
通讯作者：
Andrade, Francisco J.
中科院分区：
文献类型：
--
作者：
Guadarrama-Fernandez, Leonor;Novell, Marta;Andrade, Francisco J.
提出了一种用于监测饮料中葡萄糖含量的新型生物传感器。使用电位法检测进行测量。工作电极是以镀铂纸为载体，以含有葡萄糖氧化酶的聚乙烯醇和壳聚糖混合物制成的生物相容聚合物膜为识别层。该系统基于对酶反应产生的过氧化氢的检测，该反应以高灵敏度、选择性和简单的方式进行。该生物传感器具有良好的分析性能(灵敏度在119.6.0 3~1.0 mm范围内为-6.4 mV/dec，检测下限为0.0 2 mm)。介绍了市售橙汁中葡萄糖的测定方法。这些结果与传统的标准方法进行了验证，显示出良好的准确度和快速的分析响应。本文介绍的方法不需要复杂的样品处理，为传统方法提供了一种替代方法，特别是对于在没有实验室基础设施的情况下以最少的专业知识进行的测定。
A new biosensor for monitoring glucose levels in beverages is presented. The measurements are performed using potentiometric detection. Working electrodes are made using platinised paper as support and a biocompatible polymeric membrane made of a mixture of polyvinyl alcohol and chitosan containing glucose oxidase as the recognition layer. The system is based on the detection of the hydrogen peroxide generated by an enzymatic reaction performed in a highly sensitive, selective and simple way. The biosensors display suitable analytical performance (sensitivity -119.6 +/- 6.4 mV/dec in the 0.03-1.0 mM range with a limit of detection of 0.02 mM). Determination of glucose in commercial orange juices is presented. These results were validated against conventional standard methods, showing good accuracy and fast analytical response. The methodology presented herein does not require complex samples treatment, offering an alternative to conventional methods, particularly for determinations performed with minimal expertise and without a laboratory infrastructure.