Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75.
Molecular characterization and complete genome sequence of avian paramyxovirus type 4 prototype strain duck/Hong Kong/D3/75.
复制标题
分子表征和鸟类帕托病毒4型原型型鸭/香港/D3/75的完整基因组序列。
DOI：
10.1186/1743-422x-5-124
复制
发表时间：
2008-10-20
期刊：
影响因子：
4.8
通讯作者：
Samal, Siba K.
中科院分区：
文献类型：
--
作者：
Nayak, Baibaswata;Kumar, Sachin;Collins, Peter L.;Samal, Siba K.
禽副粘病毒（Avian paramyxoviruses，APMV）是世界范围内常见的家禽和野鸟病毒。所有的APMV，除了禽偏肺病毒，被分类在副粘病毒科的Avulavirus属中。目前，禽流感病毒属的APMV分为9个血清型（APMV 1-9）。纽卡斯尔病病毒代表APMV-1，是所有APMV类型中最具特征的。关于APMV 2-9的分子特征和致病性知之甚少。作为了解APMV-4分子遗传学和致病性的第一步，我们对APMV-4株duck/Hong Kong/D3/75进行了全基因组测序，并确定了其在鸡胚中的致病性。APMV-4的基因组长度为15，054个核苷酸（nt），符合“六位法则”。该基因组包含顺序为3 '-N-P/V-M-F-HN-L-5'的六个非重叠基因。这些基因的两侧是高度保守的转录起始和终止信号，并且具有长度从9到42 nt不等的基因间序列。该基因组在3'端含有一个55 nt的前导区。5'端尾区为17 nt，是副粘病毒科中最短的。从P基因转录的mRNA的分析表明，35%的转录本通过在编辑位点插入一个非模板化的G残基进行编辑，导致产生V mRNA。没有检测到含有两个非模板化G残基插入的信息，表明W mRNA在APMV-4感染的细胞中无效地产生。F蛋白的切割位点（DIPQR↓F）与普遍存在的细胞内蛋白酶弗林蛋白酶的优选切割位点不一致。然而，外源蛋白酶不需要在细胞培养物中的APMV-4的生长，表明切割不依赖于弗林蛋白酶位点。对副粘病毒科所有五个属的病毒的核苷酸序列的系统发育分析表明，APMV-4与APMV的关系比与其他副粘病毒的关系更密切，加强了所有APMV在副粘病毒科Avulavirus属中的分类。
Avian paramyxoviruses (APMVs) are frequently isolated from domestic and wild birds throughout the world. All APMVs, except avian metapneumovirus, are classified in the genus Avulavirus of the family Paramyxoviridae. At present, the APMVs of genus Avulavirus are divided into nine serological types (APMV 1–9). Newcastle disease virus represents APMV-1 and is the most characterized among all APMV types. Very little is known about the molecular characteristics and pathogenicity of APMV 2–9. As a first step towards understanding the molecular genetics and pathogenicity of APMV-4, we have sequenced the complete genome of APMV-4 strain duck/Hong Kong/D3/75 and determined its pathogenicity in embryonated chicken eggs. The genome of APMV-4 is 15,054 nucleotides (nt) in length, which is consistent with the "rule of six". The genome contains six non-overlapping genes in the order 3'-N-P/V-M-F-HN-L-5'. The genes are flanked on either side by highly conserved transcription start and stop signals and have intergenic sequences varying in length from 9 to 42 nt. The genome contains a 55 nt leader region at 3' end. The 5' trailer region is 17 nt, which is the shortest in the family Paramyxoviridae. Analysis of mRNAs transcribed from the P gene showed that 35% of the transcripts were edited by insertion of one non-templated G residue at an editing site leading to production of V mRNAs. No message was detected that contained insertion of two non-templated G residues, indicating that the W mRNAs are inefficiently produced in APMV-4 infected cells. The cleavage site of the F protein (DIPQR↓F) does not conform to the preferred cleavage site of the ubiquitous intracellular protease furin. However, exogenous proteases were not required for the growth of APMV-4 in cell culture, indicating that the cleavage does not depend on a furin site. Phylogenic analysis of the nucleotide sequences of viruses of all five genera of the family Paramyxoviridae showed that APMV-4 is more closely related to the APMVs than to other paramyxoviruses, reinforcing the classification of all APMVs in the genus Avulavirus of the family Paramyxoviridae.