CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients
CircRNA Expression Profile in Early-Stage Lung Adenocarcinoma Patients
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早期肺腺癌患者的 CircRNA 表达谱
DOI：
10.1159/000485953
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发表时间：
2017-01-01
影响因子：
--
通讯作者：
Xu, Ming
中科院分区：
文献类型：
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作者：
Zhao, Jiangmin;Li, Le;Xu, Ming
背景/目标：肺腺癌是一种晚期致死率高的非小细胞肺癌，在女性、不吸烟者或从不吸烟者甚至年轻人中越来越常见。目前，仍没有有效的早期诊断方法，使患者得到及时治愈。环状RNA（circular RNA，circRNA）是哺乳动物细胞中一类稳定、保守的非编码RNA，广泛参与肿瘤的发生、发展过程。然而，关于哪些特异性circRNA参与早期肺腺癌的发展仍然是一个谜。研究方法：采用高通量的circRNA芯片技术，对4例早期肺腺癌患者的癌组织及癌旁正常组织中circRNA的表达进行研究。通过生物信息学分析筛选差异表达的circRNA。进行qRT-PCR和测序以确保微阵列数据。结果：肺癌组织中共有357个circRNA表达异常，提示其在肺癌中的潜在作用。qRT-PCR检测结果显示，筛选出的5个circRNA与微阵列数据一致，并预测了circRNA-miRNA之间可能的相互作用。结论：这项工作表明，circRNA簇在早期肺腺癌中异常表达，这可能为这种疾病的早期诊断和肺癌的新遗传见解提供潜在的靶点。
Background/Aims: Lung adenocarcinoma, a form of non-small cell lung cancer with high lethality at an advanced stage, is becoming more common in women, non- or never-smokers, and even young adults. At present, there are still no effective early diagnosis methods for patients to be cured in a timely manner. Circular RNAs (circRNAs), which are stable and conserved non-coding RNA in mammalian cells, have been reported to be widely involved in the processes of cancer disease. However, it is still a puzzle as to which specific circRNAs are involved in the development of early-stage lung adenocarcinoma. Methods: Tumor samples and paired adjacent normal tissues from 4 patients with early-stage lung adenocarcinoma were selected to investigate the expression profile of circRNAs by using a high-throughput circRNA microarray. Bioinformatic analyses were conducted to screen those differentially expressed circRNAs. qRT-PCR and sequencing were performed to assure the microarray data. Results: A total of 357 circRNAs were dysregulated in the tumor samples, which suggests potential roles in lung cancer. qRT-PCR detection showed that five selected circRNAs were identical to the microarray data, and the potential circRNA-miRNA interactions were predicted. Conclusion: This work illustrates that clusters of circRNAs are aberrantly expressed in early-stage lung adenocarcinoma, which might offer potential targets for the early diagnosis of this disease and new genetic insights into lung cancer.