Genetic and antigenic variability of human respiratory syncytial virus (groups A and B) isolated over seven consecutive seasons in Argentina (1995 to 2001)
Genetic and antigenic variability of human respiratory syncytial virus (groups A and B) isolated over seven consecutive seasons in Argentina (1995 to 2001)
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DOI：
10.1128/jcm.43.5.2266-2273.2005
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发表时间：
2005-05-01
影响因子：
9.4
通讯作者：
Carballal, G
中科院分区：
文献类型：
--
作者：
Galiano, MC;Palomo, C;Carballal, G
通过 G 基因的部分核苷酸测序和抗 G 单克隆抗体的酶联免疫吸附测定分析，评估了 1995 年至 2001 年在布宜诺斯艾利斯分离的人呼吸道合胞病毒 (HRSV) 株的遗传和抗原变异性。系统发育分析显示，37 个 A 组菌株聚类为 5 个基因型，而 20 个 B 组菌株聚类为 3 个基因型。 A 组比 B 组表现出更多的遗传变异性。观察到基因型和抗原模式之间存在密切相关性。在两组病毒的 G 蛋白中检测到的变化包括 (i) 氨基酸取代和 (ii) 由于终止密码子使用或序列重复的变化而导致的蛋白质长度差异。 1999 年的 3 个 B 菌株表现出 20 个氨基酸的重复，而 2001 年的 1 个 B 菌株则有 2 个氨基酸的重复。讨论了不同流行期间阿根廷 HRSV 毒株与其他地理区域分离到的病毒之间的比较。
The genetic and antigenic variability of human respiratory syncytial virus (HRSV) strains isolated in Buenos Aires from 1995 to 2001 was evaluated by partial nucleotide sequencing of the G gene and enzyme-linked immunosorbent assay analysis with anti-G monoclonal antibodies. Phylogenetic analyses showed that 37 group A strains clustered into five genotypes, whereas 20 group B strains clustered into three genotypes. Group A showed more genetic variability than group B. A close correlation between genotypes and antigenic patterns was observed. Changes detected in the G protein of viruses from both groups included (i) amino acid substitutions and(ii) differences in protein length due to either changes in stop codon usage or sequence duplications. Three B strains from 1999 exhibited a duplication of 20 amino acids, while one B strain from 2001 had 2 amino acids duplicated. The comparison among Argentinean HRSV strains and viruses isolated in other geographical areas during different epidemics is discussed.