CURVATURE CORRECTION FACTORS IN THE MEASUREMENT OF CELL-SURFACE AREAS IN PLANT-TISSUES
CURVATURE CORRECTION FACTORS IN THE MEASUREMENT OF CELL-SURFACE AREAS IN PLANT-TISSUES
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DOI：
10.1093/jxb/34.1.87
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发表时间：
1983-01-01
影响因子：
6.9
通讯作者：
THAIN, JF
中科院分区：
文献类型：
--
作者：
THAIN, JF
估算植物组织中细胞表面积的最简单和最可靠的方法是测量组织薄切片中细胞轮廓周长的总长度。该方法由于忽略了细胞表面的曲率而产生误差。曲率的影响可以通过将轮廓周长的测量长度乘以适当的曲率校正因子来补偿。这些曲率修正系数，这是显着大的值。这些校正因子的测量在植物组织中的细胞表面积的应用进行了讨论，特别是参考叶叶肉组织。还讨论了组织中切片的适当取向的选择。
The simplest and most reliable method for the estimation of the surface area of cells in plant tissues involves the measurement of the total length of the perimeters of cell profiles in thin sections of the tissue. The method suffers from errors due to neglect of curvature of the cell surfaces. The effects of the curvature can be compensated for by multiplying the measured length of profile perimeter by appropriate curvature correction factors. Values for these curvature correction factors, which are significantly large are presented. The application of these correction factors to the measurement of cell surface areas in plant tissues is discussed with particular reference to leaf mesophyll tissue. The choice of appropriate orientations of the section in a tissue is also discussed.