INCREASED PHOSPHORYLATION OF CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II AND ITS ENDOGENOUS SUBSTRATES IN THE INDUCTION OF LONG-TERM POTENTIATION
INCREASED PHOSPHORYLATION OF CA2+/CALMODULIN-DEPENDENT PROTEIN-KINASE-II AND ITS ENDOGENOUS SUBSTRATES IN THE INDUCTION OF LONG-TERM POTENTIATION
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DOI：
10.1074/jbc.270.11.6119
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发表时间：
1995-03-17
影响因子：
4.8
通讯作者：
MIYAMOTO, E
中科院分区：
文献类型：
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作者：
FUKUNAGA, K;MULLER, D;MIYAMOTO, E
海马切片的CA 1区中长时程增强的诱导与Ca 2 +/钙调蛋白依赖性蛋白激酶II（CaM激酶II）的活性增加有关（Fukunaga，K.，斯托皮尼湖Miyamoto，E.，和Muller，D.（1993）J.Biol.Chem.268，7863-7867），在此我们报道了对P-32标记的切片的CA 1区域中的两组传入神经施加高频而非低频刺激导致该酶的两种主要底物突触蛋白I和微管相关蛋白2的磷酸化，以及CaM激酶II的自磷酸化。此外，免疫印迹分析显示，长时程增强诱导与同一区域中CaM激酶II量的增加有关。当在N-甲基-D-天冬氨酸受体拮抗剂D-2-氨基-B-膦酰基戊酸存在下施加高频刺激时，所有这些变化都被阻止。这些结果表明，CaM激酶II的激活参与了突触后和突触前区域突触增强的诱导，
Induction of long term potentiation in the CA1 region of hippocampal slices is associated with increased activity of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) (Fukunaga, K., Stoppini, L., Miyamoto, E., and Muller, D. (1993) J. Biol. Chem. 268, 7863-7867), Here we report that application of high but not low frequency stimulation to two groups of afferents in the CA1 region of P-32-labeled slices resulted in the phosphorylation of two major substrates of this enzyme, synapsin I and microtubule-associated protein 2, as well as in the autophosphorylation of CaM kinase II, Furthermore, immunoblotting analysis revealed that long term potentiation induction was associated with an increase in the amount of CaM kinase II in the same region. All these changes were prevented when high frequency stimulation was applied in the presence of the N-methyl-D-aspartate receptor antagonist, D-2-amino-B-phosphonopentanoate. These results indicate that activation of CaM kinase II is involved in the induction of synaptic potentiation in both the postsynaptic and presynaptic regions,