Biochemical and genetic analysis of pre-stalk specific acid phosphatase in Dictyostelium.
Biochemical and genetic analysis of pre-stalk specific acid phosphatase in Dictyostelium.
复制标题
盘基网柄菌茎前特异性酸性磷酸酶的生化和遗传分析。
DOI：
10.1016/0012-1606(84)90216-1
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
Kuspa,A
中科院分区：
文献类型：
--
作者：
Loomis,WF;Kuspa,A
酸性磷酸酶同工酶AP 2是盘基网柄藻蛞蝓发育过程中前柄细胞唯一已知的酶标记。还有另一种酸性磷酸酶同工酶，AP 1，存在于营养细胞中，没有细胞类型特异性。我们对这些同工酶进行了纯化，发现它们在K和热稳定性方面存在差异。这两种同工酶都受到单一位点acpA突变的影响。在theacpA位点的两个突变废除了所有的活动，AP 1和AP 2，而第三个突变降低了活性，改变了两种同工酶的热稳定性。acpA可能是AP 1和AP 2的结构基因。AP 2的细胞类型特异性似乎是由于前孢子和前柄细胞之间acpA基因产物修饰的差异造成的。AP2的差异为前茎细胞提供了一个有用的标记。
The only known enzymatic marker of pre-stalk cells at the slug stage of development inDictyostelium discoideumis an isozyme of acid phosphatase, AP2. There is another isozyme of acid phosphatase, AP1, which is present in vegetative cells and is not cell-type specific. We have purified these isozymes and find they differ inKmand thermostability. Both isozymes are affected by mutations in a single locus,acpA. Two mutations in theacpA locus abolished all activity of both AP1 and AP2 while a third mutation reduced the activity and altered the thermostability of both isozymes. It is likely thatacpA is the structural gene responsible for both AP1 and AP2. The cell-type specificity of AP2 appears to result from differences in the modification of theacpA gene product between pre-spore and pre-stalk cells. The resulting difference in AP2 provides a useful marker for pre-stalk cells.