Dual modality fluorescence confocal and spectral-domain optical coherence tomography microendoscope.
Dual modality fluorescence confocal and spectral-domain optical coherence tomography microendoscope.
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DOI：
10.1364/boe.2.000634
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发表时间：
2011-02-22
影响因子：
3.4
通讯作者：
Gmitro AF
中科院分区：
文献类型：
--
作者：
Makhlouf H;Rouse AR;Gmitro AF
光学活组织检查通过提供临床环境中组织的实时原位视图，方便了活体疾病的诊断。荧光共聚焦显微内窥镜和光学相干断层扫描(OCT)是在这方面显示出巨大潜力的两种方法。这些技术提供了互补的观点。与共聚焦系统相关的高分辨率和对比度允许在生物组织的薄层中对亚细胞细节和细胞组织进行面部可视化。OCT提供了横截面图像，显示了组织的微结构，其深度超出了共焦系统的范围。我们提出了一种新的台式成像系统的设计，它将共焦和OCT模式合并到同一光学序列中，从而允许在两种成像技术之间进行快速切换。使用简单体模的初步结果表明，通过基于光纤束的成像系统，可以同时实现共焦显微内窥镜和OCT。
Optical biopsy facilitates in vivo disease diagnoses by providing a real-time in situ view of tissue in a clinical setting. Fluorescence confocal microendoscopy and optical coherence tomography (OCT) are two methods that have demonstrated significant potential in this context. These techniques provide complementary viewpoints. The high resolution and contrast associated with confocal systems allow en face visualization of sub-cellular details and cellular organization within a thin layer of biological tissue. OCT provides cross-sectional images showing the tissue micro-architecture to a depth beyond the reach of confocal systems. We present a novel design for a bench-top imaging system that incorporates both confocal and OCT modalities in the same optical train allowing the potential for rapid switching between the two imaging techniques. Preliminary results using simple phantoms show that it is possible to realize both confocal microendoscopy and OCT through a fiber bundle based imaging system.