Pasticcino2 is a protein tyrosine phosphatase-like involved in cell proliferation and differentiation in Arabidopsis
Pasticcino2 is a protein tyrosine phosphatase-like involved in cell proliferation and differentiation in Arabidopsis
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DOI：
10.1046/j.1365-313x.2002.01456.x
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发表时间：
2002-12-01
期刊：
影响因子：
7.2
通讯作者：
Faure, JD
中科院分区：
文献类型：
--
作者：
Bellec, Y;Harrar, Y;Faure, JD
Pasticcino2(Pas2)突变体表现出胚胎和幼苗发育受损，与细胞去分化和增殖有关。在细胞分裂素的存在下，这一过程被特别地增强，导致幼苗顶端部分的愈伤组织样结构。细胞增殖是指分裂细胞的局部随机结节。在缺乏细胞分裂素的情况下，细胞增殖会导致茎上的小愈伤组织，但最常见的情况是，细胞增殖与生殖器后器官融合有关。用定位克隆的方法鉴定了PAS2基因。PAS2在植物各器官中均有表达，且不受PAS1和PAS3基因的调控。PAS2编码蛋白酪氨酸磷酸酶样家族(Ptpl)的拟南芥成员，这是一个最初在小鼠和人类中描述的新的PTP家族，其特征是PTP活性位点突变。这个蛋白质家族有一种酵母同系物，对细胞存活至关重要。酵母PAS2同源物的缺失可以被拟南芥PAS2蛋白在功能上取代，这表明PAS2功能在高等和低等真核生物之间是保守的。
The pasticcino2 (pas2) mutant shows impaired embryo and seedling development associated with cell dedifferentiation and proliferation. This process is specifically enhanced in presence of cytokinins leading to callus-like structure of the apical part of the seedling. Cell proliferation concerns localized and stochastic nodules of dividing cells. In absence of cytokinins, cell proliferation leads to small calli on stems but, most often, cell proliferation is associated with post-genital organ fusion. The PAS2 gene was identified by positional cloning. PAS2 expression was found in every plant organ and was not regulated by PAS1 and PAS3 genes. PAS2 encodes the Arabidopsis member of the protein tyrosine phosphatase-like (Ptpl) family, a new PTP family originally described in mice and humans and characterized by a mutated PTP active site. This family of proteins has a yeast homolog that is essential for cell viability. The absence of yeast PAS2 homolog can be functionally replaced by the Arabidopsis PAS2 protein, demonstrating that PAS2 function is conserved between higher and lower eukaryotes.