What Is on the Outside Matters-Surface Charge and Dissolve Organic Matter Association Affect the Toxicity and Physiological Mode of Action of Polystyrene Nanoplastics to C. elegans.
What Is on the Outside Matters-Surface Charge and Dissolve Organic Matter Association Affect the Toxicity and Physiological Mode of Action of Polystyrene Nanoplastics to C. elegans.
复制标题
DOI：
10.1021/acs.est.0c07121
复制
发表时间：
2021-04
影响因子：
11.4
通讯作者：
C. Schultz;S. Bart;E. Lahive;D. Spurgeon
中科院分区：
文献类型：
--
作者：
C. Schultz;S. Bart;E. Lahive;D. Spurgeon
为了更好地理解纳米塑料的作用，需要建立表面功能化和溶解有机物生态电晕形成的潜力，以改变不同纳米塑料的作用和毒性机制。在这里，我们评估了不同的表面电荷修饰功能化（阳性（+ve）胺化;中性非功能化;阴性（-ve）羧基化）如何改变50和60 nm聚苯乙烯纳米塑料对线虫线虫的毒性。对存活、生长和繁殖的影响的效力按+ve胺化>中性未官能化的羧基化的B-ve的顺序降低，其中+ve带电形式的毒性比-ve带电形式的毒性高>60倍。毒代动力学-毒代动力学建模（DEBtox）表明，电荷相关效价主要与效应阈值和剂量相关损伤参数的差异有关，而与毒代动力学参数无关。这表明表面功能化可能会改变纳米塑料与膜和细胞器相互作用的性质，从而导致毒性的变化。生态冠的形成降低了所有纳米塑料的毒性，表明有机分子协会可以钝化表面。在粒子之间，生态电晕相互作用导致更等效的效果;然而，即使有这些变化，带电形式的效力顺序仍被保留。这些结果对未来分组方法的发展具有重要意义。
To better understand nanoplastic effects, the potential for surface functionalization and dissolve organic matter eco-corona formation to modify the mechanisms of action and toxicity of different nanoplastics needs to be established. Here, we assess how different surface charges modifying functionalization (postive (+ve) aminated; neutral unfunctionalized; negative (-ve) carboxylated) altered the toxicity of 50 and 60 nm polystyrene nanoplastics to the nematode Caenorhabditis elegans. The potency for effects on survival, growth, and reproduction reduced in the order +ve aminated > neutral unfunctionalized ≫ -ve carboxylated with toxicity >60-fold higher for the +ve than -ve charged forms. Toxicokinetic-toxicodynamic modeling (DEBtox) showed that the charge-related potency was primarily linked to differences in effect thresholds and dose-associated damage parameters, rather than to toxicokinetic parameters. This suggests that surface functionalization may change the nature of nanoplastic interactions with membrane and organelles leading to variations in toxicity. Eco-corona formation reduced the toxicity of all nanoplastics indicating that organic molecule associations may passivate surfaces. Between particles, eco-corona interactions resulting in more equivalent effects; however, even despite these changes, the order of potency of the charged forms was retained. These results have important implications for the development of future grouping approaches.