Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking
Extended Dual-Focus Microscopy for Ratiometric-Based 3D Movement Tracking
复制标题
用于基于比率的3D运动跟踪的扩展双焦点显微镜
DOI：
10.3390/app10186243
复制
发表时间：
2020-09-01
影响因子：
2.7
通讯作者：
Higuchi, Hideo
中科院分区：
文献类型：
--
作者：
Lee, Seohyun;Kim, Hyuno;Higuchi, Hideo
对活细胞中小细胞器的三维运动进行成像可以为生物医学学科中药物输送和病毒传播的动力学提供关键信息。为了使用显微镜稳定地监测这样的细胞内运动，同时需要沿光轴沿着的长景深和精确的三维跟踪。在目前的工作中，我们提出了一个扩展的双焦点光学显微镜系统相结合的双焦平面成像方案和物镜透镜振荡，这使得精确定位的长轴向范围。所提出的系统利用高分辨率的功能，通过连接每个轴向成像水平采集的部分校准结果，保持比率法的实际优势。
Imaging the three-dimensional movement of small organelles in living cells can provide key information for the dynamics of drug delivery and virus transmission in biomedical disciplines. To stably monitor such intracellular motion using microscope, long depth of field along optical axis and accurate three-dimensional tracking are simultaneously required. In the present work, we suggest an extended dual-focus optics microscopy system by combining a bifocal plane imaging scheme and objective lens oscillation, which enables accurate localization for a long axial range. The proposed system exploits high-resolution functionality by concatenating partial calibration result acquired each axial imaging level, maintaining the practical advantages of ratiometric method.