PURIFIED ASTROCYTES PROMOTE THE INVITRO DIVISION OF A BIPOTENTIAL GLIAL PROGENITOR-CELL
PURIFIED ASTROCYTES PROMOTE THE INVITRO DIVISION OF A BIPOTENTIAL GLIAL PROGENITOR-CELL
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DOI：
10.1002/j.1460-2075.1984.tb02122.x
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发表时间：
1984-01-01
期刊：
影响因子：
11.4
通讯作者：
MURRAY, K
中科院分区：
文献类型：
--
作者：
NOBLE, M;MURRAY, K
新生大鼠的视神经含有双能神经胶质祖细胞，其可通过组织培养条件诱导分化成少突胶质细胞（CNS的髓鞘形成细胞）或2型星形胶质细胞（仅在CNS的有髓鞘束中发现的星形胶质细胞群）。在以前的研究中，大多数少突胶质细胞2型星形胶质细胞（O-2A）祖细胞在体外3天内分化，祖细胞或其分化后代的分裂相对较少。O-2A祖细胞在培养物中被纯化的1型星形胶质细胞群刺激分裂，1型星形胶质细胞是在大鼠视神经中发现的另一种胶质细胞类型。这种细胞-细胞相互作用似乎是由可溶性因子介导的，并导致大量祖细胞和少突胶质细胞的产生。当少突胶质细胞开始在体内大量产生时，1型星形胶质细胞是视神经中主要的胶质细胞类型，该星形胶质细胞亚群可能在正常发育过程中扩大少突胶质细胞群中发挥重要作用。
Optic nerves of neonatal rats contain a bipotential glial progenitor cell which can be induced by tissue culture conditions to differentiate into either an oligodendrocyte (the myelin-forming cell of the CNS) or a type 2 astrocyte (an astrocyte population found only in the myelinated tracts of the CNS). In previous studies most oligodendrocyte-type 2 astrocyte (O-2A) progenitor cells differentiated within 3 days in vitro with relatively little division of the progenitors or their differentiated progeny. The O-2A progenitors are stimulated to divide in culture by purified populations of type 1 astrocytes, another glial cell-type found in the rat optic nerve. This cell-cell interaction appears to be mediated by a soluble factor(s) and results in the production of large numbers of both progenitor cells and oligodendrocytes. As type 1 astrocytes are the major glial cell-type in the optic nerve when oligodendrocytes first begin to be produced in large numbers in vivo, this astrocyte subpopulation may play an important role in expanding the oligodendrocyte population during normal development.