The role of Capon in multiple myeloma
The role of Capon in multiple myeloma
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DOI：
10.1177/1010428317713674
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发表时间：
2017-06
期刊：
影响因子：
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通讯作者：
Yaodong Shen;Hai-yan Liu;S. Gu;Ziwei Wei;Hong Liu
中科院分区：
文献类型：
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作者：
Yaodong Shen;Hai-yan Liu;S. Gu;Ziwei Wei;Hong Liu
Capon是一氧化氮合酶1的配体蛋白。最近，研究表明，Capon参与了肿瘤的发展。在这一过程中，它独立于一氧化氮合酶1的调节。同时，研究发现一氧化氮合酶1在多发性骨髓瘤中表达，但其在骨髓瘤发生发展中的作用尚不清楚。在本研究中，我们发现Capon在正常的多发性骨髓瘤细胞和贴壁的多发性骨髓瘤细胞中有不同的表达。在骨髓瘤细胞增殖过程中，Capon的表达降低，减少了细胞周期在G1期的阻滞，促进了骨髓瘤细胞的增殖。细胞粘附介导的耐药是影响多发性骨髓瘤化疗疗效的重要因素之一。如果Capon的表达降低，则骨髓瘤细胞粘附于纤连蛋白或骨髓基质细胞（骨髓间充质干细胞）。此外，在粘附于骨髓间充质干细胞的骨髓瘤细胞系中沉默Capon后，细胞系对化疗剂的敏感性降低。我们还发现，Capon的表达减少导致AKT信号通路的激活。这些结果可能有助于研究Capon在多发性骨髓瘤中的作用。
Capon is a ligand protein of nitric oxide synthase 1. Recently, studies have shown that Capon is involved in the development of tumors. It is independent of the regulation of nitric oxide synthase 1 in this process. At the same time, studies have found that nitric oxide synthase 1 is expressed in multiple myeloma, but its role in the development and progression of myeloma remains unclear. In this study, we found that there was a different expression of Capon between the normal multiple myeloma cells and the adherent multiple myeloma cells. In the process of myeloma cell proliferation, the reduced expression of Capon reduces the arrest of the cell cycle in the G1 phase and promotes the proliferation of myeloma cells. Cell adhesion–mediated drug resistance is one of the most important factors, which affect the chemotherapy effect of multiple myeloma. If the expression of Capon is decreased, myeloma cells are adhered to fibronectin or bone marrow stromal cells (bone marrow mesenchymal stem cells). In addition, the sensitivity of the cell line to chemotherapeutic agents was reduced after silencing Capon in the myeloma cell line which was adhered to bone marrow mesenchymal stem cells. We also found that reduced expression of Capon resulted in the activation of the AKT signaling pathway. In conclusion, these results may be helpful in studying the role of Capon in multiple myeloma.