IDENTIFICATION OF ATTENUATING MUTATIONS ON THE REOVIRUS TYPE-3 S1 DOUBLE-STRANDED-RNA SEGMENT WITH A RAPID SEQUENCING TECHNIQUE
IDENTIFICATION OF ATTENUATING MUTATIONS ON THE REOVIRUS TYPE-3 S1 DOUBLE-STRANDED-RNA SEGMENT WITH A RAPID SEQUENCING TECHNIQUE
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DOI：
10.1128/jvi.60.1.64-67.1986
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发表时间：
1986-10-01
影响因子：
5.4
通讯作者：
FIELDS, BN
中科院分区：
文献类型：
--
作者：
BASSELDUBY, R;SPRIGGS, DR;FIELDS, BN
在.sigma.1蛋白的主要中和结构域中具有突变的呼肠孤病毒3型变体已减弱神经毒力并限制向神经性。我们设计了一种快速 RNA 测序技术的变体，以促进双链 RNA 的分析。我们对 5 种减毒 3 型呼肠孤病毒变种的 S1 双链 RNA 片段（编码 .sigma.1 蛋白）进行了测序。其中四个变体在密码子 419 处发生变化，第五个变体在密码子 340 处发生变化，所有这些都导致 .sigma.1 蛋白中的氨基酸取代。我们在 3 型呼肠孤病毒 .sigma.1 蛋白上发现了两个在神经毒力中发挥关键作用的位点。
Reovirus type 3 variants with mutations in the major neutralization domain of the .sigma.1 protein have attenuated neurovirulence and restricted neurotropism. We devised a variation of the rapid RNA sequencing technique to facilitate the analysis of double-stranded RNA. We sequenced the S1 double-stranded RNA segment, which encodes the .sigma.1 protein, of five attenuated reovirus type 3 variants. Four of the variants have changes in codon 419, and a fifth variant has a change at codon 340, all of which resulted in amino acid substitutions in the .sigma.1 protein. We identified two sites on the reovirus type 3 .sigma.1 protein that play a critical role in neurovirulence.