Potential of a novel polyherbal formulation BASANT for prevention of Chlamydia trachomatis infection
Potential of a novel polyherbal formulation BASANT for prevention of Chlamydia trachomatis infection
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DOI：
10.1016/j.ijantimicag.2008.02.010
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发表时间：
2008-07-01
影响因子：
10.8
通讯作者：
Mittal, A.
中科院分区：
文献类型：
--
作者：
Bhengraj, A. R.;Dar, Sajad A.;Mittal, A.
研究了一种新型中草药制剂巴斯汀对沙眼衣原体的抑制作用。体外药敏试验采用沙眼衣原体直接暴露(感染前与Basant孵育)和沙眼衣原体在HeLa 229细胞内暴露(感染后与Basant孵育)。标准血清型D/UW-3/CX在12、30、60 mU g/m L的浓度下，分别在感染前60、30、15分钟与强毒剂孵育后均显示完全抑制作用。感染后培养48h后，8~10mU g/mL巴斯坦对标准血清型D/UW-3/CX和5株沙眼衣原体临床分离株均有完全抑制作用。巴桑特还在30微克/毫升的浓度下抑制了从一名多西环素治疗失败患者那里获得的临床分离株。对标准株和临床分离株的检测均表明，巴桑特对沙眼衣原体具有一定的抗菌活性，提示巴桑特在预防沙眼衣原体经性途径感染方面具有潜在的临床应用价值。(C)2008年Elsevier B.V.和国际化疗学会。版权所有。
The effect of a novel polyherbal formulation BASANT on Chlamydia trachomatis was studied. In vitro sensitivity testing was done by direct exposure of C. trachomatis (pre-infection incubation with BASANT) and exposure of C. trachomatis within HeLa 229 cells (post-infection incubation with BASANT). Pre-infection incubation of standard serovar D/UW-3/Cx with BASANT showed complete inhibition after 60, 30 and 15 min of incubation at concentrations of 12, 30 and 60 mu g/mL, respectively. In the post-infection incubation, 8-10 mu g/mL of BASANT showed complete inhibition of standard serovar D/UW-3/Cx as well as of five clinical isolates of C. trachomatis after 48 h of incubation. BASANT also inhibited a clinical isolate obtained from a doxycycline treatment failure patient at a concentration of 30 mu g/mL. Both assays with standard and clinical isolates showed that BASANT has antimicrobial activity against C. trachomatis, suggesting the potential clinical utility of BASANT for the prevention of C. trachomatis infection by the sexual route. (c) 2008 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.