RNAS WITH DUAL-SPECIFICITY AND DUAL RNAS WITH SIMILAR SPECIFICITY
RNAS WITH DUAL-SPECIFICITY AND DUAL RNAS WITH SIMILAR SPECIFICITY
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DOI：
10.1126/science.7513905
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发表时间：
1994-05-20
期刊：
影响因子：
56.9
通讯作者：
YARUS, M
中科院分区：
文献类型：
--
作者：
CONNELL, GJ;YARUS, M
RNA的生物学作用由其可能的反应限定，可以通过选择来探索。结合一种配体的选定RNA与结合两种相关配体的RNA的比较表明，单个核苷酸取代可以扩大结合特异性。具有双重（联合）特异性的RNA位点具有腺嘌呤和胞嘧啶碱基，其pK（a）向上移动，从而模拟有效的一般酸碱催化剂。联合网站还包含两个保守的，环状精氨酸编码三联体涉及精氨酸网站的形成。两个选定的联合RNA在某些区域是相同的，而在其他区域是不同的。不同的区域，像一些肽，似乎功能相似，但一级结构不相似。
The biological role of RNA is delimited by its possible reactions, which can be explored by selection. A comparison of selected RNAs that bind one ligand with those that bind two related ligands suggests that a single nucleotide substitution can expand binding specificity. An RNA site with dual (joint) specificity has adenine and cytosine bases whose pK(a)'s appear shifted upward, thereby mimicking an efficient general acid-base catalyst. The joint site also contains two conserved, looped arginine-coding triplets implicated in arginine site formation. Two selected joint RNAs are identical in some regions and distinct in others. The distinct regions, like some peptides, seem to function similarly without being similar in primary structure.