Wheat ferritins: Improving the iron content of the wheat grain
Wheat ferritins: Improving the iron content of the wheat grain
复制标题
DOI：
10.1016/j.jcs.2012.03.005
复制
发表时间：
2012-09-01
影响因子：
3.8
通讯作者：
Holm, Preben Bach
中科院分区：
文献类型：
--
作者：
Borg, Soren;Brinch-Pedersen, Henrik;Holm, Preben Bach
小麦铁蛋白全补体的表征表明，现代六倍体小麦基因组包含两个铁蛋白基因 TaFer1 和 TaFer2，每个基因由三个同源等位基因代表，分别位于 5 号和 4 号染色体上。这两个基因的调控和表达存在差异。除在胚乳中外，TaFer1 基因表达最丰富，并受铁和脱落酸状态的调节。与 TaFer2 相比，TaFer1 的启动子具有铁和 ABA 响应元件，支持表达数据。 TaFer1 和 TaFer2 基因编码两种同工型，可能功能不同，并且在谷物中铁蛋白的杂聚物结构中发挥作用。小麦籽粒的铁生物强化是可能的。 TaFer1-A 基因的胚乳靶向基因内过度表达导致籽粒中铁含量提高 50-85%。 (C) 2012 Elsevier Ltd. 保留所有权利。
The characterization of the full complement of wheat ferritins show that the modern hexaploid wheat genome contains two ferritin genes, TaFer1 and TaFer2, each represented by three homeoalleles and placed on chromosome 5 and 4, respectively. The two genes are differentially regulated and expressed. The TaFer1 genes are, except in the endosperm, the most abundantly expressed and regulated by iron and abscisic acid status. The promoter of TaFer1, in contrast to TaFer2, has iron- and ABA-responsive elements, supporting the expression data. The TaFer1 and TaFer2 genes encode two isoforms, probably functional different and acting in heteropolymer structures of ferritin in cereals. Iron biofortification of the wheat grain is possible. Endosperm targeted intragenic overexpressing of the TaFer1-A gene results in a 50-85% higher iron content in the grain. (C) 2012 Elsevier Ltd. All rights reserved.